| http://purl.uniprot.org/citations/16125341 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16125341 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16125341 | http://www.w3.org/2000/01/rdf-schema#comment | "The 79 kD gonadotropin-regulated testicular long chain acyl-CoA synthetase gene (GR-LACS) is a hormone-regulated member of the acyl-CoA synthetase family that is expressed abundantly in Leydig cells and to a lesser extent in germinal cells of the adult testis. GR-LACS possesses an ATP/AMP binding domain and the fatty acyl-CoA synthetase (FACS) signature motif. To gain insights into the transcriptional regulation of GR-LACS in gonadal cells, we determined the genomic organization of the gene, including the upstream flanking sequences. The mouse GR-LACS gene spans over at least 45 kb and the coding region is encoded by exons 1-14. All exon-intron junction sites correspond to the consensus splice sequence GT-AG. Exon 7 and 11 comprise the conserved ATP/AMP binding domain and the FACS signature motif, respectively. Primer extension and S1 nuclease analyses demonstrated four transcriptional start sites located at -266/-216 bp 5' to the ATG codon. The minimal promoter domain resides within -254/-217 bp 5' to ATG codon, and upstream sequences to -404 bp (-1035/-405 bp) contribute to the inhibition of transcription in the expressing mouse Leydig tumor cells. Removal of -217/-1 bp, containing a 23 nt GC rich sequence (-112/-90) with an Sp1/Sp3 binding element, within the 1st exon of this TATA-less promoter, significantly reduced GR-LACS gene transcription. Transcriptional activity was abolished by a 2 nt mutation of this element. Thus, functional analyses of this promoter domain indicate that transcription of GR-LACS gene requires an Sp1/Sp3 binding element downstream of the transcriptional start sites which is essential for basal promoter activity."xsd:string |
| http://purl.uniprot.org/citations/16125341 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.gene.2005.07.006"xsd:string |
| http://purl.uniprot.org/citations/16125341 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.gene.2005.07.006"xsd:string |
| http://purl.uniprot.org/citations/16125341 | http://purl.uniprot.org/core/author | "Li J."xsd:string |
| http://purl.uniprot.org/citations/16125341 | http://purl.uniprot.org/core/author | "Li J."xsd:string |
| http://purl.uniprot.org/citations/16125341 | http://purl.uniprot.org/core/author | "Sheng Y."xsd:string |
| http://purl.uniprot.org/citations/16125341 | http://purl.uniprot.org/core/author | "Sheng Y."xsd:string |
| http://purl.uniprot.org/citations/16125341 | http://purl.uniprot.org/core/author | "Dufau M.L."xsd:string |
| http://purl.uniprot.org/citations/16125341 | http://purl.uniprot.org/core/author | "Dufau M.L."xsd:string |
| http://purl.uniprot.org/citations/16125341 | http://purl.uniprot.org/core/author | "Tsai-Morris C.-H."xsd:string |
| http://purl.uniprot.org/citations/16125341 | http://purl.uniprot.org/core/author | "Tsai-Morris C.-H."xsd:string |
| http://purl.uniprot.org/citations/16125341 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/16125341 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/16125341 | http://purl.uniprot.org/core/name | "Gene"xsd:string |
| http://purl.uniprot.org/citations/16125341 | http://purl.uniprot.org/core/name | "Gene"xsd:string |
| http://purl.uniprot.org/citations/16125341 | http://purl.uniprot.org/core/pages | "20-26"xsd:string |
| http://purl.uniprot.org/citations/16125341 | http://purl.uniprot.org/core/pages | "20-26"xsd:string |
| http://purl.uniprot.org/citations/16125341 | http://purl.uniprot.org/core/title | "The gonadotropin-regulated long-chain acyl CoA synthetase gene: a novel downstream Sp1/Sp3 binding element critical for transcriptional promoter activity."xsd:string |
| http://purl.uniprot.org/citations/16125341 | http://purl.uniprot.org/core/title | "The gonadotropin-regulated long-chain acyl CoA synthetase gene: a novel downstream Sp1/Sp3 binding element critical for transcriptional promoter activity."xsd:string |
| http://purl.uniprot.org/citations/16125341 | http://purl.uniprot.org/core/volume | "360"xsd:string |
| http://purl.uniprot.org/citations/16125341 | http://purl.uniprot.org/core/volume | "360"xsd:string |
| http://purl.uniprot.org/citations/16125341 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16125341 |
| http://purl.uniprot.org/citations/16125341 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16125341 |