| http://purl.uniprot.org/citations/16155256 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16155256 | http://www.w3.org/2000/01/rdf-schema#comment | "Cyclin G-associated kinase (GAK/auxilin 2), the ubiquitous form of the neuronal-specific protein auxilin 1, is an essential cofactor for the Hsc70-dependent uncoating of clathrin-coated vesicles. We have now investigated the effect of knocking down GAK in HeLa cells by vector-based small hairpin RNA. Functionally, depletion of GAK caused a marked decrease in internalization of both transferrin and epidermal growth factor and altered mannose 6-phosphate receptor trafficking, but had little effect on the recycling of transferrin receptor back to the plasma membrane. Structurally, depletion of GAK caused a marked reduction in perinuclear clathrin associated with the trans-Golgi network and in the number of clathrin-coated pits on the plasma membrane, and reduced clathrin exchange on the few clathrin-coated pits that remained. Surprisingly, while clathrin depletion does not prevent adaptors from assembling on the membrane, depletion of GAK caused a dramatic reduction in AP2 and epsin on the plasma membrane and AP1 and GGA at the trans-Golgi network. A similar effect was caused by expression of a dominant negative Hsp70 mutant. These results suggest that GAK, in conjunction with Hsc70, not only uncoats clathrin-coated vesicles and induces clathrin exchange on clathrin-coated pits, but also mediates binding of clathrin and adaptors to the plasma membrane and the trans-Golgi network."xsd:string |
| http://purl.uniprot.org/citations/16155256 | http://purl.org/dc/terms/identifier | "doi:10.1242/jcs.02548"xsd:string |
| http://purl.uniprot.org/citations/16155256 | http://purl.uniprot.org/core/author | "Lee D.W."xsd:string |
| http://purl.uniprot.org/citations/16155256 | http://purl.uniprot.org/core/author | "Zhang F."xsd:string |
| http://purl.uniprot.org/citations/16155256 | http://purl.uniprot.org/core/author | "Zhao X."xsd:string |
| http://purl.uniprot.org/citations/16155256 | http://purl.uniprot.org/core/author | "Eisenberg E."xsd:string |
| http://purl.uniprot.org/citations/16155256 | http://purl.uniprot.org/core/author | "Greene L.E."xsd:string |
| http://purl.uniprot.org/citations/16155256 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/16155256 | http://purl.uniprot.org/core/name | "J Cell Sci"xsd:string |
| http://purl.uniprot.org/citations/16155256 | http://purl.uniprot.org/core/pages | "4311-4321"xsd:string |
| http://purl.uniprot.org/citations/16155256 | http://purl.uniprot.org/core/title | "Depletion of GAK/auxilin 2 inhibits receptor-mediated endocytosis and recruitment of both clathrin and clathrin adaptors."xsd:string |
| http://purl.uniprot.org/citations/16155256 | http://purl.uniprot.org/core/volume | "118"xsd:string |
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