| http://purl.uniprot.org/citations/16157298 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16157298 | http://www.w3.org/2000/01/rdf-schema#comment | "Neonatal rat ventricular myocytes (NRVM) grown in normoxic environment are not susceptible to Fas-induced apoptosis. In the present work, we tested the hypothesis that free radical injury represented by transient exposure to H2O2 sensitizes NRVM to Fas-mediated apoptosis. NRVM were treated with H2O2 (0.5 mM) for 2-4 h and thereafter exposed for 7 h to recombinant Fas ligand (rFasL, 10 ng/ml) plus an enhancing antibody (1 microg/ml). Apoptotic cardiomyocytes were counted and apoptosis-related proteins were measured by Western blot. H2O2 alone induced apoptosis (9.4+/-1.0%) that was preceded by activation of caspases-8 and -3, and PARP degradation. Incubation of NRVM with H2O2, followed by exposure to rFasL, increased the apoptotic index to 13.8+/-2.0%, but did not change caspase-8 or PARP activation. To investigate the mechanism underlying the sensitizing affect of H2O2 towards Fas-induced apoptosis, we studied the effects of H2O2 on the expression of key apoptosis signaling proteins. Incubation with H2O2 for 2-4 h decreased Fas expression and the expression of the Fas-related antiapoptotic proteins FLIP(L) and ARC, and increased the expression of the antiapoptotic proteins bcl-2 and xIAP. FADD expression was unchanged. Next, we tested the effect of H2O2 on the apoptosis-inducing, Fas-dependent Daxx-ASK-1-JUN kinase pathway. H2O2 dramatically increased ASK-1 expression and JUN kinase activation, but did not effect Daxx expression. Based on these findings we concluded that H2O2 sensitizes NRVM to Fas-mediated apoptosis by activating the Daxx-ASK-1-JUN kinase pathway, and by shifting the balance between proapoptotic and antiapoptotic proteins towards the former."xsd:string |
| http://purl.uniprot.org/citations/16157298 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbrc.2005.08.167"xsd:string |
| http://purl.uniprot.org/citations/16157298 | http://purl.uniprot.org/core/author | "Larisch S."xsd:string |
| http://purl.uniprot.org/citations/16157298 | http://purl.uniprot.org/core/author | "Binah O."xsd:string |
| http://purl.uniprot.org/citations/16157298 | http://purl.uniprot.org/core/author | "Shilkrut M."xsd:string |
| http://purl.uniprot.org/citations/16157298 | http://purl.uniprot.org/core/author | "Yaniv G."xsd:string |
| http://purl.uniprot.org/citations/16157298 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/16157298 | http://purl.uniprot.org/core/name | "Biochem Biophys Res Commun"xsd:string |
| http://purl.uniprot.org/citations/16157298 | http://purl.uniprot.org/core/pages | "740-746"xsd:string |
| http://purl.uniprot.org/citations/16157298 | http://purl.uniprot.org/core/title | "Hydrogen peroxide predisposes neonatal rat ventricular myocytes to Fas-mediated apoptosis."xsd:string |
| http://purl.uniprot.org/citations/16157298 | http://purl.uniprot.org/core/volume | "336"xsd:string |
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