| http://purl.uniprot.org/citations/16195535 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16195535 | http://www.w3.org/2000/01/rdf-schema#comment | "Gamma-glutamyl transpeptidase (GGT) plays critical roles in glutathione homeostasis and metabolism. Rat GGT is a single-copy gene from which seven types of GGT mRNA with a common protein encoding sequence, but different 5'-untranslated regions, may be transcribed. We previously showed that type V-2 was the predominant form of GGT mRNA in rat L2 epithelial cells, and that it could be induced by 4-hydroxynonenal (HNE) through the electrophile response element (EpRE) located in GGT promoter 5 (GP5). Here, we report transcription factors binding to GP5 EpRE and the involved signaling pathways. Immunodepletion gel shift assays demonstrated that GP5 EpRE bound JunB, c-Jun, FosB, and Fra2 from unstimulated cells, and that after exposure to HNE, EpRE binding complexes contained nuclear factor erythroid 2-related factor (Nrf) 1, Nrf2, JunB, c-Jun, FosB, c-Fos, Fra1, and Fra2. HNE-induced binding of Nrf2 and c-Jun in GP5 EpRE was confirmed by chromatin immunoprecipitation assays. Using reporter assays and specific inhibitors, we found that HNE induction of rat GGT mRNA V-2 was dependent on activation of extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinase (MAPK), but not protein kinase C or phosphatidylinositol 3-kinase. Pretreatment with ERK and p38MAPK inhibitors also blocked HNE-increased EpRE binding. HNE-increased nuclear content of Nrf1, Nrf2, and c-Jun in L2 cells was partially blocked by inhibition of either ERK1/2 or p38MAPK and completely blocked by simultaneous inhibition of both MAPKs. In conclusion, HNE induces GGT mRNA V-2 through altered EpRE transcription factor binding mediated by both ERK and p38MAPK."xsd:string |
| http://purl.uniprot.org/citations/16195535 | http://purl.org/dc/terms/identifier | "doi:10.1165/rcmb.2005-0280oc"xsd:string |
| http://purl.uniprot.org/citations/16195535 | http://purl.uniprot.org/core/author | "Liu H."xsd:string |
| http://purl.uniprot.org/citations/16195535 | http://purl.uniprot.org/core/author | "Zhang H."xsd:string |
| http://purl.uniprot.org/citations/16195535 | http://purl.uniprot.org/core/author | "Forman H.J."xsd:string |
| http://purl.uniprot.org/citations/16195535 | http://purl.uniprot.org/core/author | "Laperche Y."xsd:string |
| http://purl.uniprot.org/citations/16195535 | http://purl.uniprot.org/core/author | "Liu R.M."xsd:string |
| http://purl.uniprot.org/citations/16195535 | http://purl.uniprot.org/core/author | "Iles K.E."xsd:string |
| http://purl.uniprot.org/citations/16195535 | http://purl.uniprot.org/core/author | "Postlethwait E.M."xsd:string |
| http://purl.uniprot.org/citations/16195535 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16195535 | http://purl.uniprot.org/core/name | "Am J Respir Cell Mol Biol"xsd:string |
| http://purl.uniprot.org/citations/16195535 | http://purl.uniprot.org/core/pages | "174-181"xsd:string |
| http://purl.uniprot.org/citations/16195535 | http://purl.uniprot.org/core/title | "4-Hydroxynonenal induces rat gamma-glutamyl transpeptidase through mitogen-activated protein kinase-mediated electrophile response element/nuclear factor erythroid 2-related factor 2 signaling."xsd:string |
| http://purl.uniprot.org/citations/16195535 | http://purl.uniprot.org/core/volume | "34"xsd:string |
| http://purl.uniprot.org/citations/16195535 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16195535 |
| http://purl.uniprot.org/citations/16195535 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/16195535 |
| http://purl.uniprot.org/uniprot/P17325#attribution-81E306FCC1D9EFBB266674D4E9D639C4 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/16195535 |
| http://purl.uniprot.org/uniprot/#_P17325-mappedCitation-16195535 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16195535 |
| http://purl.uniprot.org/uniprot/P17325 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/16195535 |