| http://purl.uniprot.org/citations/16212417 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16212417 | http://www.w3.org/2000/01/rdf-schema#comment | "A biosensor-based micro-affinity purification method to recover protein binding partners and their complexes for down stream proteomics analysis has been developed using the BIAcore 3000 fitted with a prototype Surface Prep Unit (SPU). The recombinant GST-intracellular domain of E-cadherin or the recombinant GST-beta-catenin binding domain of Adenomatous Polyposis Coli (APC) were immobilized onto the SPU and used to affinity purify binding partners from chromatographically enriched SW480 colon cancer cell lysates. A GST-immobilized surface was used as a control. Samples recovered from the SPU were subjected to SDS-PAGE with sensitive Coomassie staining followed by automated in-gel digestion and LC-MS/MS. The results obtained using the SPU were compared with similar experiments performed using Sepharose beads."xsd:string |
| http://purl.uniprot.org/citations/16212417 | http://purl.org/dc/terms/identifier | "doi:10.1021/pr050132x"xsd:string |
| http://purl.uniprot.org/citations/16212417 | http://purl.uniprot.org/core/author | "Ross J."xsd:string |
| http://purl.uniprot.org/citations/16212417 | http://purl.uniprot.org/core/author | "Nice E."xsd:string |
| http://purl.uniprot.org/citations/16212417 | http://purl.uniprot.org/core/author | "Burgess A.W."xsd:string |
| http://purl.uniprot.org/citations/16212417 | http://purl.uniprot.org/core/author | "Connolly L."xsd:string |
| http://purl.uniprot.org/citations/16212417 | http://purl.uniprot.org/core/author | "Catimel B."xsd:string |
| http://purl.uniprot.org/citations/16212417 | http://purl.uniprot.org/core/author | "Rothacker J."xsd:string |
| http://purl.uniprot.org/citations/16212417 | http://purl.uniprot.org/core/author | "Catimel J."xsd:string |
| http://purl.uniprot.org/citations/16212417 | http://purl.uniprot.org/core/author | "Clippingdale A."xsd:string |
| http://purl.uniprot.org/citations/16212417 | http://purl.uniprot.org/core/author | "Faux M."xsd:string |
| http://purl.uniprot.org/citations/16212417 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/16212417 | http://purl.uniprot.org/core/name | "J Proteome Res"xsd:string |
| http://purl.uniprot.org/citations/16212417 | http://purl.uniprot.org/core/pages | "1646-1656"xsd:string |
| http://purl.uniprot.org/citations/16212417 | http://purl.uniprot.org/core/title | "Biosensor-based micro-affinity purification for the proteomic analysis of protein complexes."xsd:string |
| http://purl.uniprot.org/citations/16212417 | http://purl.uniprot.org/core/volume | "4"xsd:string |
| http://purl.uniprot.org/citations/16212417 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16212417 |
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