| http://purl.uniprot.org/citations/16219694 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16219694 | http://www.w3.org/2000/01/rdf-schema#comment | "Cenp-F is an unusual kinetochore protein in that it localizes to the nuclear matrix in interphase and the nuclear envelope at the G2/M transition; it is farnesylated and rapidly degraded after mitosis. We have recently shown that farnesylation of Cenp-F is required for G2/M progression, its localization to kinetochores, and its degradation. However, the role Cenp-F plays in mitosis has remained enigmatic. Here we show that, following repression of Cenp-F by RNA interference (RNAi), the processes of metaphase chromosome alignment, anaphase chromosome segregation and cytokinesis all fail. Although kinetochores attach to microtubules in Cenp-F-deficient cells, the oscillatory movements that normally occur following K-fibre formation are severely dampened. Consistently, inter-kinetochore distances are reduced. In addition, merotelic associations are observed, suggesting that whereas kinetochores can attach microtubules in the absence of Cenp-F, resolving inappropriate interactions is inhibited. Repression of Cenp-F does not appear to compromise the spindle checkpoint. Rather, the chromosome alignment defect induced by Cenp-F RNA interference is accompanied by a prolonged mitosis, indicating checkpoint activation. Indeed, the prolonged mitosis induced by Cenp-F RNAi is dependent on the spindle checkpoint kinase BubR1. Surprisingly, chromosomes in Cenp-F-deficient cells frequently show a premature loss of chromatid cohesion. Thus, in addition to regulating kinetochore-microtubule interactions, Cenp-F might be required to protect centromeric cohesion prior to anaphase commitment. Intriguingly, whereas most of the sister-less kinetochores cluster near the spindle poles, some align at the spindle equator, possibly through merotelic or lateral orientations."xsd:string |
| http://purl.uniprot.org/citations/16219694 | http://purl.org/dc/terms/identifier | "doi:10.1242/jcs.02614"xsd:string |
| http://purl.uniprot.org/citations/16219694 | http://purl.uniprot.org/core/author | "Taylor S.S."xsd:string |
| http://purl.uniprot.org/citations/16219694 | http://purl.uniprot.org/core/author | "Holt S.V."xsd:string |
| http://purl.uniprot.org/citations/16219694 | http://purl.uniprot.org/core/author | "Hussein D."xsd:string |
| http://purl.uniprot.org/citations/16219694 | http://purl.uniprot.org/core/author | "Allan V.J."xsd:string |
| http://purl.uniprot.org/citations/16219694 | http://purl.uniprot.org/core/author | "Wozniak M.J."xsd:string |
| http://purl.uniprot.org/citations/16219694 | http://purl.uniprot.org/core/author | "Vergnolle M.A."xsd:string |
| http://purl.uniprot.org/citations/16219694 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/16219694 | http://purl.uniprot.org/core/name | "J Cell Sci"xsd:string |
| http://purl.uniprot.org/citations/16219694 | http://purl.uniprot.org/core/pages | "4889-4900"xsd:string |
| http://purl.uniprot.org/citations/16219694 | http://purl.uniprot.org/core/title | "Silencing Cenp-F weakens centromeric cohesion, prevents chromosome alignment and activates the spindle checkpoint."xsd:string |
| http://purl.uniprot.org/citations/16219694 | http://purl.uniprot.org/core/volume | "118"xsd:string |
| http://purl.uniprot.org/citations/16219694 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16219694 |
| http://purl.uniprot.org/citations/16219694 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/16219694 |
| http://purl.uniprot.org/uniprot/#_A0A9L9PXU7-mappedCitation-16219694 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16219694 |
| http://purl.uniprot.org/uniprot/#_P49454-mappedCitation-16219694 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16219694 |
| http://purl.uniprot.org/uniprot/P49454 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/16219694 |
| http://purl.uniprot.org/uniprot/A0A9L9PXU7 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/16219694 |