| http://purl.uniprot.org/citations/16233652 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16233652 | http://www.w3.org/2000/01/rdf-schema#comment | "Sake yeasts take up gamma-aminobutyric acid (GABA) derived from rice-koji in the primary stage of sake brewing. The GABA content in sake brewed with the UGA1 disruptant, which lacked GABA transaminase, was higher than that brewed with the wild-type strain K701. The UGA1 disruptant derived from sake yeast could not grow on a medium with GABA as the sole nitrogen source. We have isolated the sake yeast mutants of K701 that were unable to grow on a medium containing GABA as the sole nitrogen source. The growth defect of GAB7-1 and GAB7-2 mutants on GABA plates was complemented by UGA1, which encodes GABA transaminase, and UGA2, which encodes succinic semialdehyde dehydrogenase (SSADH), respectively. DNA sequence analysis revealed that GAB7-1 had a homozygous nonsense mutation in UGA1 and GAB7-2 had a heterozygous mutation (G247D) in UGA2. The GABA transaminase activity of GAB7-1 and the SSADH activity of GAB7-2 were markedly lower than those of K701. These GAB mutants displayed a higher intracellular GABA content. The GABA contents in sake brewed with the mutants GAB7-1 and GAB7-2 were 2.0 and 2.1 times higher, respectively, than that brewed with the wild-type strain K701. These results suggest that the reduced function of the GABA utilization pathway increases the GABA content in sake."xsd:string |
| http://purl.uniprot.org/citations/16233652 | http://purl.org/dc/terms/identifier | "doi:10.1016/s1389-1723(04)70228-5"xsd:string |
| http://purl.uniprot.org/citations/16233652 | http://purl.uniprot.org/core/author | "Mizoguchi H."xsd:string |
| http://purl.uniprot.org/citations/16233652 | http://purl.uniprot.org/core/author | "Hara S."xsd:string |
| http://purl.uniprot.org/citations/16233652 | http://purl.uniprot.org/core/author | "Takahashi T."xsd:string |
| http://purl.uniprot.org/citations/16233652 | http://purl.uniprot.org/core/author | "Furukawa A."xsd:string |
| http://purl.uniprot.org/citations/16233652 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/16233652 | http://purl.uniprot.org/core/name | "J Biosci Bioeng"xsd:string |
| http://purl.uniprot.org/citations/16233652 | http://purl.uniprot.org/core/pages | "412-418"xsd:string |
| http://purl.uniprot.org/citations/16233652 | http://purl.uniprot.org/core/title | "Isolation and characterization of sake yeast mutants deficient in gamma-aminobutyric acid utilization in sake brewing."xsd:string |
| http://purl.uniprot.org/citations/16233652 | http://purl.uniprot.org/core/volume | "97"xsd:string |
| http://purl.uniprot.org/citations/16233652 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16233652 |
| http://purl.uniprot.org/citations/16233652 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/16233652 |
| http://purl.uniprot.org/uniprot/#_P38067-mappedCitation-16233652 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16233652 |
| http://purl.uniprot.org/uniprot/#_P17649-mappedCitation-16233652 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16233652 |
| http://purl.uniprot.org/uniprot/P17649 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/16233652 |
| http://purl.uniprot.org/uniprot/P38067 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/16233652 |