| http://purl.uniprot.org/citations/16237198 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16237198 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16237198 | http://www.w3.org/2000/01/rdf-schema#comment | "The synthesis of N-acetylneuraminate (Neu5Ac), the main form of sialic acid, proceeds in vertebrates through the condensation of N-acetylmannosamine 6-phosphate and phosphoenolpyruvate to Neu5Ac-9-phosphate, followed by the dephosphorylation of the latter by a specific phosphatase. The sequence encoding Neu5Ac-9-phosphate phosphatase (Neu5Ac-9-Pase; E.C. 3.1.3.29) has not been determined until now. In this work, we have purified Neu5Ac-9-Pase more than 1000-fold from rat liver. Its dependency on Mg2+ and the fact that it was inhibited by vanadate and Ca2+ suggested that it belonged to the haloacid dehalogenase family of phosphatases. Trypsin digestion and mass spectrometry analysis of a polypeptide of about 30 kDa that co-eluted with the activity in the last purification step indicated the presence of a protein designated "haloacid dehalogenase-like hydrolase domain containing 4." The human ortholog of this protein is encoded by a 2-exon gene present on chromosome 20p11. The human protein was overexpressed in Escherichia coli as a fusion protein with a polyHis tag and purified to homogeneity. The recombinant enzyme displayed a >230-fold higher catalytic efficiency on Neu5Ac-9-phosphate than on its second best substrate. Its properties were similar to those of the enzyme purified from rat liver. Neu5Ac inhibited the enzymatic activity by 50% at 15 mM, indicating that no significant inhibition is exerted at physiological concentrations of Neu5Ac. The identification of the gene encoding Neu5Ac-9-Pase will facilitate studies aimed at testing its potential implication in unexplained forms of glycosylation deficiency."xsd:string |
| http://purl.uniprot.org/citations/16237198 | http://purl.org/dc/terms/identifier | "doi:10.1093/glycob/cwj050"xsd:string |
| http://purl.uniprot.org/citations/16237198 | http://purl.org/dc/terms/identifier | "doi:10.1093/glycob/cwj050"xsd:string |
| http://purl.uniprot.org/citations/16237198 | http://purl.uniprot.org/core/author | "Van Schaftingen E."xsd:string |
| http://purl.uniprot.org/citations/16237198 | http://purl.uniprot.org/core/author | "Van Schaftingen E."xsd:string |
| http://purl.uniprot.org/citations/16237198 | http://purl.uniprot.org/core/author | "Vertommen D."xsd:string |
| http://purl.uniprot.org/citations/16237198 | http://purl.uniprot.org/core/author | "Vertommen D."xsd:string |
| http://purl.uniprot.org/citations/16237198 | http://purl.uniprot.org/core/author | "Delpierre G."xsd:string |
| http://purl.uniprot.org/citations/16237198 | http://purl.uniprot.org/core/author | "Delpierre G."xsd:string |
| http://purl.uniprot.org/citations/16237198 | http://purl.uniprot.org/core/author | "Maliekal P."xsd:string |
| http://purl.uniprot.org/citations/16237198 | http://purl.uniprot.org/core/author | "Maliekal P."xsd:string |
| http://purl.uniprot.org/citations/16237198 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16237198 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16237198 | http://purl.uniprot.org/core/name | "Glycobiology"xsd:string |
| http://purl.uniprot.org/citations/16237198 | http://purl.uniprot.org/core/name | "Glycobiology"xsd:string |
| http://purl.uniprot.org/citations/16237198 | http://purl.uniprot.org/core/pages | "165-172"xsd:string |
| http://purl.uniprot.org/citations/16237198 | http://purl.uniprot.org/core/pages | "165-172"xsd:string |
| http://purl.uniprot.org/citations/16237198 | http://purl.uniprot.org/core/title | "Identification of the sequence encoding N-acetylneuraminate-9-phosphate phosphatase."xsd:string |
| http://purl.uniprot.org/citations/16237198 | http://purl.uniprot.org/core/title | "Identification of the sequence encoding N-acetylneuraminate-9-phosphate phosphatase."xsd:string |
| http://purl.uniprot.org/citations/16237198 | http://purl.uniprot.org/core/volume | "16"xsd:string |
| http://purl.uniprot.org/citations/16237198 | http://purl.uniprot.org/core/volume | "16"xsd:string |
| http://purl.uniprot.org/citations/16237198 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16237198 |
| http://purl.uniprot.org/citations/16237198 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16237198 |