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http://purl.uniprot.org/citations/16244790http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/16244790http://www.w3.org/2000/01/rdf-schema#comment"

Purpose

Inflammatory breast cancer (IBC) is the most aggressive form of locally advanced breast cancer (LABC). The IBC phenotype is characterized by an infiltrative growth pattern, increased (lymph)angiogenesis and the propensity to invade dermal lymphatics. In pancreatic cancer, interactions between caveolin-1 and RhoC GTPase, a key molecule in causing the IBC phenotype, regulate tumour cell motility and invasion. In this study we sought to investigate the role of caveolin-1 and -2 in IBC cell lines and in human IBC samples.

Experimental design

Differential methylation techniques identified the methylation status of the caveolin-1 and -2 promoters in human mammary epithelial cells (HMECs) and the SUM149 cell line. In cell line experiments, caveolin-1 and -2 mRNA and protein expression were compared in HMECs, MCF10A, the SUM102 non-IBC cell lines and 2 IBC cell lines (SUM149 and SUM190). Furthermore, caveolin-1 and -2 mRNA and protein expression were compared in human IBC and non-IBC samples using cDNA microarray, real-time qRT-PCR and immunohistochemistry. Results were correlated with RhoC protein expression data.

Results

In the SUM149 cell line, the caveolin-1 and -2 promoter sites were hypomethylated. A significantly increased expression of caveolin-1 and -2, both at the mRNA and protein level was found in IBC cell lines and in human samples of IBC: caveolin-1 and -2 mRNA were respectively 1.7 (p = 0.02) and 2.2 (p = 0.03) fold more expressed in IBC compared to non IBC and at the protein level, 41.4% of IBC specimens expressed either caveolin-1 or -2, compared to 15.6% of non-IBC specimens (p = 0.03). Furthermore a correlation was found between RhoC protein expression and caveolin-1 (p = 0.1) or caveolin-2 (p = 0.09) or either caveolin-1 or -2 protein expression (p = 0.04).

Conclusions

Although considered a tumour suppressor in breast cancer, we demonstrated overexpression of caveolin-1 and -2 in IBC cell lines and in human samples of IBC, most likely due to hypomethylation of their respective promoters. These results confirm the distinct molecular signature of IBC. Our data further suggest interaction between RhoC GTPase and the caveolins in IBC."xsd:string
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http://purl.uniprot.org/citations/16244790http://purl.uniprot.org/core/author"Merajver S.D."xsd:string
http://purl.uniprot.org/citations/16244790http://purl.uniprot.org/core/author"van Golen K.L."xsd:string
http://purl.uniprot.org/citations/16244790http://purl.uniprot.org/core/author"van Dam P."xsd:string
http://purl.uniprot.org/citations/16244790http://purl.uniprot.org/core/author"Dirix L.Y."xsd:string
http://purl.uniprot.org/citations/16244790http://purl.uniprot.org/core/author"Vermeulen P.B."xsd:string
http://purl.uniprot.org/citations/16244790http://purl.uniprot.org/core/author"Van Laere S.J."xsd:string
http://purl.uniprot.org/citations/16244790http://purl.uniprot.org/core/author"Van Marck E.A."xsd:string
http://purl.uniprot.org/citations/16244790http://purl.uniprot.org/core/author"Van den Eynden G.G."xsd:string
http://purl.uniprot.org/citations/16244790http://purl.uniprot.org/core/author"Van der Auwera I."xsd:string
http://purl.uniprot.org/citations/16244790http://purl.uniprot.org/core/date"2006"xsd:gYear
http://purl.uniprot.org/citations/16244790http://purl.uniprot.org/core/name"Breast Cancer Res Treat"xsd:string
http://purl.uniprot.org/citations/16244790http://purl.uniprot.org/core/pages"219-228"xsd:string
http://purl.uniprot.org/citations/16244790http://purl.uniprot.org/core/title"Overexpression of caveolin-1 and -2 in cell lines and in human samples of inflammatory breast cancer."xsd:string
http://purl.uniprot.org/citations/16244790http://purl.uniprot.org/core/volume"95"xsd:string
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