| http://purl.uniprot.org/citations/16262243 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16262243 | http://www.w3.org/2000/01/rdf-schema#comment | "Human acidic fibroblast growth factor (hFGF-1) is a potent mitogen and is involved in the regulation of key cellular process such as angiogenesis, differentiation, and morphogenesis. hFGF-1 is a signal peptide-less protein that is released into the extracellular compartment as a multiprotein complex consisting of S100A13, synaptotagmin (Syt1), and a hFGF-1 homodimer. Cu(2+) is known to play an important role in the formation of the multiprotein release complex. The source of Cu(2+) required for the formation of the multiprotein release complex is not clear. In this study, we show that the cytoplasmic C2A domain of synaptotagmin binds to Cu(2+) ions with high affinity. Results from the isothermal calorimetry (ITC), near-UV circular dichroism (CD), and absorption spectroscopy experiments suggest that four Cu(2+) ions bind per molecule of C2A domain. Far-UV CD and limited trypsin digestion analysis reveal that the C2A domain undergoes a mild conformational change upon binding to Cu(2+). Competition experiments monitored by ITC and fluorescence resonance energy transfer indicate that Cu(2+) and Ca(2+) ions share common binding sites on the C2A domain. Cu(2+) ions compete with and replace Ca(2+) ions bound to the C2A domain. Two-dimensional nuclear magnetic resonance spectroscopy data clearly show that Cu(2+) ions bind to the Ca(2+) binding sites in the loops (loops 1-3) located at the apex of the structure of the C2A domain. In addition, there is a unique Cu(2+) binding site located in the loop connecting beta-strands 7 and 8. It appears that the C2A domain provides the Cu(2+) ions required for the formation of the multiprotein FGF release complex."xsd:string |
| http://purl.uniprot.org/citations/16262243 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi051387r"xsd:string |
| http://purl.uniprot.org/citations/16262243 | http://purl.uniprot.org/core/author | "Yu C."xsd:string |
| http://purl.uniprot.org/citations/16262243 | http://purl.uniprot.org/core/author | "Kumar T.K."xsd:string |
| http://purl.uniprot.org/citations/16262243 | http://purl.uniprot.org/core/author | "Rajalingam D."xsd:string |
| http://purl.uniprot.org/citations/16262243 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/16262243 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/16262243 | http://purl.uniprot.org/core/pages | "14431-14442"xsd:string |
| http://purl.uniprot.org/citations/16262243 | http://purl.uniprot.org/core/title | "The C2A domain of synaptotagmin exhibits a high binding affinity for copper: implications in the formation of the multiprotein FGF release complex."xsd:string |
| http://purl.uniprot.org/citations/16262243 | http://purl.uniprot.org/core/volume | "44"xsd:string |
| http://purl.uniprot.org/citations/16262243 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16262243 |
| http://purl.uniprot.org/citations/16262243 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/16262243 |
| http://purl.uniprot.org/uniprot/#_A6IGE2-mappedCitation-16262243 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16262243 |
| http://purl.uniprot.org/uniprot/#_P21707-mappedCitation-16262243 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16262243 |
| http://purl.uniprot.org/uniprot/#_Q707P0-mappedCitation-16262243 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16262243 |
| http://purl.uniprot.org/uniprot/#_Q707P1-mappedCitation-16262243 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16262243 |
| http://purl.uniprot.org/uniprot/#_Q707P2-mappedCitation-16262243 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16262243 |
| http://purl.uniprot.org/uniprot/#_Q707P4-mappedCitation-16262243 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16262243 |
| http://purl.uniprot.org/uniprot/P21707 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/16262243 |
| http://purl.uniprot.org/uniprot/Q707P1 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/16262243 |
| http://purl.uniprot.org/uniprot/Q707P4 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/16262243 |
| http://purl.uniprot.org/uniprot/Q707P0 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/16262243 |
| http://purl.uniprot.org/uniprot/A6IGE2 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/16262243 |
| http://purl.uniprot.org/uniprot/Q707P2 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/16262243 |