| http://purl.uniprot.org/citations/16280322 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16280322 | http://www.w3.org/2000/01/rdf-schema#comment | "The peroxin Pex19p comprising 299 amino acids functions in peroxisomal membrane assembly. We here developed a cell-free system for transport of membrane proteins to peroxisomes. Pex19p interacts with multiple membrane peroxins, including other membrane biogenesis peroxins, Pex16p and Pex26p, involved in matrix protein import. Cell-free synthesized, 35S-labeled Pex19p was targeted to subcellular fractions containing peroxisomes from Chinese hamster ovary-K1 cells as well as peroxisomes isolated from rat liver in an ATP-dependent manner. Such translocation was also reproduced with in vitro synthesized 35S-Pex16p with two transmembrane segments and C-tail anchor-type 35S-Pex26p, upon incubation with 35S-Pex19p in the reaction mixtures containing isolated peroxisomes. The transported 35S-Pex16p and 35S-Pex26p were integrated into membranes as assessed by the sodium carbonate extraction method. Peroxisome-associated and partly Na2CO3-resistant 35S-Pex19p was released to the cytosolic fraction upon incubation in the absence of ATP, whereas 35S-Pex16p and 35S-Pex26p remained in the membranes. Furthermore, not only 35S-Pex19p but also 35S-Pex19p complexes each with 35S-Pex16p and 35S-Pex26p were bound to 35S-Pex3p in vitro. Together, these results strongly suggested that Pex19p translocates the membrane peroxins from the cytosol to peroxisomes in an ATP- and Pex3p-dependent manner and then shuttles back to the cytosol."xsd:string |
| http://purl.uniprot.org/citations/16280322 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m509819200"xsd:string |
| http://purl.uniprot.org/citations/16280322 | http://purl.uniprot.org/core/author | "Fujiki Y."xsd:string |
| http://purl.uniprot.org/citations/16280322 | http://purl.uniprot.org/core/author | "Matsuzono Y."xsd:string |
| http://purl.uniprot.org/citations/16280322 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16280322 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/16280322 | http://purl.uniprot.org/core/pages | "36-42"xsd:string |
| http://purl.uniprot.org/citations/16280322 | http://purl.uniprot.org/core/title | "In vitro transport of membrane proteins to peroxisomes by shuttling receptor Pex19p."xsd:string |
| http://purl.uniprot.org/citations/16280322 | http://purl.uniprot.org/core/volume | "281"xsd:string |
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