| http://purl.uniprot.org/citations/16280551 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16280551 | http://www.w3.org/2000/01/rdf-schema#comment | "Colony-stimulating factor 1 (CSF-1) is an important physiological chemoattractant for macrophages. The mechanisms by which CSF-1 elicits the formation of filamentous actin (F-actin)-rich membrane protrusions and induces macrophage migration are not fully understood. In particular, very little is known regarding the contribution of the different members of the Wiskott-Aldrich Syndrome protein (WASP) family of actin regulators in response to CSF-1. Although a role for WASP itself in macrophage chemotaxis has been previously identified, no data was available regarding the function of WASP family verprolin-homologous (WAVE) proteins in this cell type. We found that WAVE2 was the predominant isoform to be expressed in primary macrophages and in cells derived from the murine monocyte/macrophage RAW264.7 cell line (RAW/LR5). CSF-1 treatment of macrophages resulted in WAVE2 accumulation in F-actin-rich protrusions induced by CSF-1. Inhibition of WAVE2 function by expressing a dominant-negative mutant or introducing anti-WAVE2 antibodies in RAW/LR5 cells, as well as reduction of endogenous WAVE2 expression by RNA-mediated interference (RNAi), resulted in a significant reduction of CSF-1-elicited F-actin protrusions. WAVE2 was found in a protein complex together with Abelson kinase interactor 1 (Abi1) in resting or stimulated cells. Both WAVE2 and Abi1 were recruited to and necessary for the formation of F-actin protrusions in response to CSF-1. Reducing the levels of WAVE2, directly or by targeting Abi1, resulted in an impaired cell migration to CSF-1. Altogether these data identify a WAVE2-Abi1 complex crucial for the normal actin cytoskeleton reorganization and migration of macrophages in response to CSF-1."xsd:string |
| http://purl.uniprot.org/citations/16280551 | http://purl.org/dc/terms/identifier | "doi:10.1242/jcs.02638"xsd:string |
| http://purl.uniprot.org/citations/16280551 | http://purl.uniprot.org/core/author | "Cox D."xsd:string |
| http://purl.uniprot.org/citations/16280551 | http://purl.uniprot.org/core/author | "Yamaguchi H."xsd:string |
| http://purl.uniprot.org/citations/16280551 | http://purl.uniprot.org/core/author | "Isaac B."xsd:string |
| http://purl.uniprot.org/citations/16280551 | http://purl.uniprot.org/core/author | "Gevrey J.C."xsd:string |
| http://purl.uniprot.org/citations/16280551 | http://purl.uniprot.org/core/author | "Kheir W.A."xsd:string |
| http://purl.uniprot.org/citations/16280551 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/16280551 | http://purl.uniprot.org/core/name | "J Cell Sci"xsd:string |
| http://purl.uniprot.org/citations/16280551 | http://purl.uniprot.org/core/pages | "5369-5379"xsd:string |
| http://purl.uniprot.org/citations/16280551 | http://purl.uniprot.org/core/title | "A WAVE2-Abi1 complex mediates CSF-1-induced F-actin-rich membrane protrusions and migration in macrophages."xsd:string |
| http://purl.uniprot.org/citations/16280551 | http://purl.uniprot.org/core/volume | "118"xsd:string |
| http://purl.uniprot.org/citations/16280551 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16280551 |
| http://purl.uniprot.org/citations/16280551 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/16280551 |
| http://purl.uniprot.org/uniprot/#_B7ZCU0-mappedCitation-16280551 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16280551 |
| http://purl.uniprot.org/uniprot/#_B7ZCU3-mappedCitation-16280551 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16280551 |
| http://purl.uniprot.org/uniprot/#_B7ZCU4-mappedCitation-16280551 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16280551 |
| http://purl.uniprot.org/uniprot/#_Q8BH43-mappedCitation-16280551 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16280551 |
| http://purl.uniprot.org/uniprot/#_Q8BZD7-mappedCitation-16280551 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16280551 |
| http://purl.uniprot.org/uniprot/#_Q3TJR5-mappedCitation-16280551 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16280551 |
| http://purl.uniprot.org/uniprot/#_Q3TJ64-mappedCitation-16280551 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16280551 |
| http://purl.uniprot.org/uniprot/#_Q3TMI0-mappedCitation-16280551 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16280551 |
| http://purl.uniprot.org/uniprot/#_Q3T9Z7-mappedCitation-16280551 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16280551 |
| http://purl.uniprot.org/uniprot/#_Q3TPY5-mappedCitation-16280551 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16280551 |