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http://purl.uniprot.org/citations/16291869http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/16291869http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/16291869http://www.w3.org/2000/01/rdf-schema#comment"

Objectives

Over a 3 year period (2000-2003) 21 Escherichia coli, 5 Klebsiella pneumoniae, 1 Serratia marcescens and 1 Proteus mirabilis producing CTX-M-type beta-lactamase were collected from five different hospitals in Paris, France. This study was conducted to analyse the genetic environment of these 28 bla(CTX-M) genes.

Methods

Antimicrobial susceptibility testing was performed by the disc diffusion method and MICs of various beta-lactams were determined by an agar dilution method. PCR was used to detect and sequence alleles encoding CTX-M, TEM, SHV and CMY enzymes. The genetic environment was analysed by amplification and direct sequencing using various set of PCR primers or cloning in pBK-CMV.

Results

Sequence analysis revealed that these isolates contained seven different bla(CTX-M) genes: bla(CTX-M-1) (4 strains), bla(CTX-M-2) (2 strains), bla(CTX-M-3) (4 strains), bla(CTX-M-9) (1 strain), bla(CTX-M-14) (5 strains), bla(CTX-M-15) (11 strains), bla(CTX-M-24) (1 strain). TEM-1 was associated with CTX-M-type enzymes in 15 isolates. Two strains produced both CTX-M-15 and SHV-2 or CTX-M-14 and CMY-2. In 25 strains the insertion sequence ISEcp1 was located upstream of the 5' end of the bla(CTX-M) gene. Among these strains, in five isolates, ISEcp1 was disrupted by insertion sequences such as IS26 (in three of them) or IS1 or IS10. Insertion sequence IS903 was found downstream of bla(CTX-M-14) or bla(CTX-M-24). Examination of the other three bla(CTX-M) genes (two bla(CTX-M-2) and one bla(CTX-M-9)) by cloning, sequencing and PCR analysis revealed the presence of complex Class 1 integrons, In35, an integron similar to In60 and a novel integron.

Conclusions

This work further confirmed the predominant role of ISEcp1 in the mobilization of bla(CTX-M) genes of the CTX-M-1 cluster and the presence of In35, of an integron similar to In60 and a novel complex Class 1 integron."xsd:string
http://purl.uniprot.org/citations/16291869http://purl.org/dc/terms/identifier"doi:10.1093/jac/dki398"xsd:string
http://purl.uniprot.org/citations/16291869http://purl.org/dc/terms/identifier"doi:10.1093/jac/dki398"xsd:string
http://purl.uniprot.org/citations/16291869http://purl.uniprot.org/core/author"Eckert C."xsd:string
http://purl.uniprot.org/citations/16291869http://purl.uniprot.org/core/author"Eckert C."xsd:string
http://purl.uniprot.org/citations/16291869http://purl.uniprot.org/core/author"Arlet G."xsd:string
http://purl.uniprot.org/citations/16291869http://purl.uniprot.org/core/author"Arlet G."xsd:string
http://purl.uniprot.org/citations/16291869http://purl.uniprot.org/core/author"Gautier V."xsd:string
http://purl.uniprot.org/citations/16291869http://purl.uniprot.org/core/author"Gautier V."xsd:string
http://purl.uniprot.org/citations/16291869http://purl.uniprot.org/core/date"2006"xsd:gYear
http://purl.uniprot.org/citations/16291869http://purl.uniprot.org/core/date"2006"xsd:gYear
http://purl.uniprot.org/citations/16291869http://purl.uniprot.org/core/name"J. Antimicrob. Chemother."xsd:string
http://purl.uniprot.org/citations/16291869http://purl.uniprot.org/core/name"J Antimicrob Chemother"xsd:string
http://purl.uniprot.org/citations/16291869http://purl.uniprot.org/core/pages"14-23"xsd:string
http://purl.uniprot.org/citations/16291869http://purl.uniprot.org/core/pages"14-23"xsd:string
http://purl.uniprot.org/citations/16291869http://purl.uniprot.org/core/title"DNA sequence analysis of the genetic environment of various blaCTX-M genes."xsd:string
http://purl.uniprot.org/citations/16291869http://purl.uniprot.org/core/title"DNA sequence analysis of the genetic environment of various blaCTX-M genes."xsd:string
http://purl.uniprot.org/citations/16291869http://purl.uniprot.org/core/volume"57"xsd:string
http://purl.uniprot.org/citations/16291869http://purl.uniprot.org/core/volume"57"xsd:string
http://purl.uniprot.org/citations/16291869http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/16291869
http://purl.uniprot.org/citations/16291869http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/16291869
http://purl.uniprot.org/citations/16291869http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/16291869
http://purl.uniprot.org/citations/16291869http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/16291869
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