| http://purl.uniprot.org/citations/16316992 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16316992 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16316992 | http://www.w3.org/2000/01/rdf-schema#comment | "Many G protein-coupled receptors possess carboxyl-terminal motifs ideal for interaction with PDZ scaffold proteins, which can control receptor trafficking and signaling in a cell-specific manner. To gain a panoramic view of beta1-adrenergic receptor (beta AR) interactions with PDZ scaffolds, the beta1AR carboxyl terminus was screened against a newly developed proteomic array of PDZ domains. These screens confirmed beta1AR associations with several previously identified PDZ partners, such as PSD-95, MAGI-2, GIPC, and CAL. Moreover, two novel beta1AR-interacting proteins, SAP97 and MAGI-3, were also identified. The beta1AR carboxyl terminus was found to bind specifically to the first PDZ domain of MAGI-3, with the last four amino acids (E-S-K-V) of beta1AR being the key determinants of the interaction. Full-length beta1AR robustly associated with full-length MAGI-3 in cells, and this association was abolished by mutation of the beta1AR terminal valine residue to alanine (V477A), as determined by co-immunoprecipitation experiments and immunofluorescence co-localization studies. MAGI-3 co-expression with beta1AR profoundly impaired beta1AR-mediated ERK1/2 activation but had no apparent effect on beta1AR-mediated cyclic AMP generation or agonist-promoted beta1AR internalization. These findings revealed that the interaction of MAGI-3 with beta1AR can selectively regulate specific aspects of receptor signaling. Moreover, the screens of the PDZ domain proteomic array provide a comprehensive view of beta1AR interactions with PDZ scaffolds, thereby shedding light on the molecular mechanisms by which beta1 AR signaling and trafficking can be regulated in a cell-specific manner."xsd:string |
| http://purl.uniprot.org/citations/16316992 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m509503200"xsd:string |
| http://purl.uniprot.org/citations/16316992 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m509503200"xsd:string |
| http://purl.uniprot.org/citations/16316992 | http://purl.uniprot.org/core/author | "He J."xsd:string |
| http://purl.uniprot.org/citations/16316992 | http://purl.uniprot.org/core/author | "He J."xsd:string |
| http://purl.uniprot.org/citations/16316992 | http://purl.uniprot.org/core/author | "Inuzuka H."xsd:string |
| http://purl.uniprot.org/citations/16316992 | http://purl.uniprot.org/core/author | "Inuzuka H."xsd:string |
| http://purl.uniprot.org/citations/16316992 | http://purl.uniprot.org/core/author | "Yang X."xsd:string |
| http://purl.uniprot.org/citations/16316992 | http://purl.uniprot.org/core/author | "Yang X."xsd:string |
| http://purl.uniprot.org/citations/16316992 | http://purl.uniprot.org/core/author | "Xu J."xsd:string |
| http://purl.uniprot.org/citations/16316992 | http://purl.uniprot.org/core/author | "Xu J."xsd:string |
| http://purl.uniprot.org/citations/16316992 | http://purl.uniprot.org/core/author | "Xiong Y."xsd:string |
| http://purl.uniprot.org/citations/16316992 | http://purl.uniprot.org/core/author | "Xiong Y."xsd:string |
| http://purl.uniprot.org/citations/16316992 | http://purl.uniprot.org/core/author | "Bellini M."xsd:string |
| http://purl.uniprot.org/citations/16316992 | http://purl.uniprot.org/core/author | "Bellini M."xsd:string |
| http://purl.uniprot.org/citations/16316992 | http://purl.uniprot.org/core/author | "Castleberry A.M."xsd:string |
| http://purl.uniprot.org/citations/16316992 | http://purl.uniprot.org/core/author | "Castleberry A.M."xsd:string |
| http://purl.uniprot.org/citations/16316992 | http://purl.uniprot.org/core/author | "Hall R.A."xsd:string |
| http://purl.uniprot.org/citations/16316992 | http://purl.uniprot.org/core/author | "Hall R.A."xsd:string |
| http://purl.uniprot.org/citations/16316992 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16316992 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16316992 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/16316992 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |