| http://purl.uniprot.org/citations/16318585 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16318585 | http://www.w3.org/2000/01/rdf-schema#comment | "Regulation of gene transcription by the cytokines tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma) involves complex interactions between NF-kappaB and Stat families of transcription factors. The purpose of this study was to identify the spatial promoter requirements that govern cytokine synergy for gene transcription regulated by NF-kappaB and Stat factors. Using a set of transcription reporter-luciferase constructs, we show that the relative orientation of juxtaposed NF-kappaB-Stat (SIE) cis-elements determines the ability of TNF-alpha and IFN-gamma to induce gene transcription. Further, NF-kappaB and Stat1 proteins directly regulate transcription by interacting cooperatively on NF-kappaB-SIE DNA binding in response to TNF-alpha plus IFN-gamma. Coimmunoprecipitation provides evidence for a direct NF-kappaB/Stat1 protein-protein interaction. In contrast, IFN-gamma inhibits TNF-alpha-induced transcription of an NF-kappaB reporter gene in a Stat1-dependent mechanism in 2fTGH fibroblasts. Similarly, Stat1 is inhibitory to NF-kappaB overexpression-induced transcription. IFN-gamma and Stat1-dependent inhibition of NF-kappaB transcription occurs independent of TNF-alpha-induced NF-kappaB DNA binding. Interestingly, IFN-gamma pretreatment of 2fTGH fibroblasts potentiates TNF-alpha induction of Stat1 DNA binding. Further, ChIP analysis was applied to detect cytokine-induced in vivo binding and transcriptional regulation of the human inducible nitric oxide synthase (iNOS) gene by NF-kappaB and Stat1. These data demonstrate complex transcriptional regulatory mechanisms elicited by TNF-alpha and IFN-gamma and have potentially important implications for other genes differentially controlled by cytokines."xsd:string |
| http://purl.uniprot.org/citations/16318585 | http://purl.org/dc/terms/identifier | "doi:10.1089/jir.2005.25.707"xsd:string |
| http://purl.uniprot.org/citations/16318585 | http://purl.uniprot.org/core/author | "Guo Z."xsd:string |
| http://purl.uniprot.org/citations/16318585 | http://purl.uniprot.org/core/author | "Shao L."xsd:string |
| http://purl.uniprot.org/citations/16318585 | http://purl.uniprot.org/core/author | "Geller D.A."xsd:string |
| http://purl.uniprot.org/citations/16318585 | http://purl.uniprot.org/core/author | "Ganster R.W."xsd:string |
| http://purl.uniprot.org/citations/16318585 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/16318585 | http://purl.uniprot.org/core/name | "J Interferon Cytokine Res"xsd:string |
| http://purl.uniprot.org/citations/16318585 | http://purl.uniprot.org/core/pages | "707-719"xsd:string |
| http://purl.uniprot.org/citations/16318585 | http://purl.uniprot.org/core/title | "Differential effects of TNF-alpha and IFN-gamma on gene transcription mediated by NF-kappaB-Stat1 interactions."xsd:string |
| http://purl.uniprot.org/citations/16318585 | http://purl.uniprot.org/core/volume | "25"xsd:string |
| http://purl.uniprot.org/citations/16318585 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16318585 |
| http://purl.uniprot.org/citations/16318585 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/16318585 |
| http://purl.uniprot.org/uniprot/#_A0A7R8GUN6-mappedCitation-16318585 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16318585 |
| http://purl.uniprot.org/uniprot/#_A0A0G2YPN5-mappedCitation-16318585 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16318585 |
| http://purl.uniprot.org/uniprot/#_A0A1U9X8M9-mappedCitation-16318585 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16318585 |
| http://purl.uniprot.org/uniprot/#_A0A411D318-mappedCitation-16318585 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16318585 |
| http://purl.uniprot.org/uniprot/#_A0A510GAH8-mappedCitation-16318585 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16318585 |
| http://purl.uniprot.org/uniprot/#_A0A510GDC6-mappedCitation-16318585 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16318585 |
| http://purl.uniprot.org/uniprot/#_A5D905-mappedCitation-16318585 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16318585 |
| http://purl.uniprot.org/uniprot/#_A0A510GFL8-mappedCitation-16318585 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16318585 |
| http://purl.uniprot.org/uniprot/#_C1K3N5-mappedCitation-16318585 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16318585 |
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| http://purl.uniprot.org/uniprot/#_B5BUQ6-mappedCitation-16318585 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16318585 |