| http://purl.uniprot.org/citations/1632457 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1632457 | http://www.w3.org/2000/01/rdf-schema#comment | "Human mesangial cells secrete tissue-type plasminogen activator (t-PA) and plasminogen activator inhibitor 1 (PAI-1), the latter being secreted in large excess in vitro. We demonstrate that PAI-1 is a major component of the extracellular matrix of cultured human mesangial cells, where its deposition is dependent on cell density. By immunogold silver staining, epipolarization microscopy and dispersive X-ray spectrometry, we have shown that matrix-associated PAI-1 is synthesized by spreading human mesangial cells, as indicated by the time-dependent accumulation of PAI-1 and the inhibitory effect of cycloheximide. Furthermore, by in situ hybridization, PAI-1 mRNA was detected in cultured mesangial cells. t-PA is present inside the cells, or at the cell surface, but is never associated with the extracellular matrix. Exogenous t-PA can remove matrix-associated PAI-1 without affecting cell adhesion. A similar effect was obtained by addition of urokinase-type plasminogen activator (u-PA) but not with fibrinolysis unrelated enzymes. In conclusion, PAI-1 is synthesized by human cultured mesangial cells and is deposited in the extracellular matrix by nonconfluent cells, whereas less PAI-1 is seen between confluent cells. This can explain the absence of detectable PAI-1 in normal human kidney biopsies. t-PA released by mesangial cells can bind and detach matrix PAI-1."xsd:string |
| http://purl.uniprot.org/citations/1632457 | http://purl.uniprot.org/core/author | "Schleuning W.D."xsd:string |
| http://purl.uniprot.org/citations/1632457 | http://purl.uniprot.org/core/author | "Delarue F."xsd:string |
| http://purl.uniprot.org/citations/1632457 | http://purl.uniprot.org/core/author | "Adida C."xsd:string |
| http://purl.uniprot.org/citations/1632457 | http://purl.uniprot.org/core/author | "Rondeau E."xsd:string |
| http://purl.uniprot.org/citations/1632457 | http://purl.uniprot.org/core/author | "Hagege J."xsd:string |
| http://purl.uniprot.org/citations/1632457 | http://purl.uniprot.org/core/author | "Medcalf R."xsd:string |
| http://purl.uniprot.org/citations/1632457 | http://purl.uniprot.org/core/author | "Peraldi M.N."xsd:string |
| http://purl.uniprot.org/citations/1632457 | http://purl.uniprot.org/core/author | "Sraer J.D."xsd:string |
| http://purl.uniprot.org/citations/1632457 | http://purl.uniprot.org/core/date | "1992"xsd:gYear |
| http://purl.uniprot.org/citations/1632457 | http://purl.uniprot.org/core/name | "Am J Pathol"xsd:string |
| http://purl.uniprot.org/citations/1632457 | http://purl.uniprot.org/core/pages | "117-128"xsd:string |
| http://purl.uniprot.org/citations/1632457 | http://purl.uniprot.org/core/title | "Plasminogen activator inhibitor-1 deposition in the extracellular matrix of cultured human mesangial cells."xsd:string |
| http://purl.uniprot.org/citations/1632457 | http://purl.uniprot.org/core/volume | "141"xsd:string |
| http://purl.uniprot.org/citations/1632457 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/1632457 |
| http://purl.uniprot.org/citations/1632457 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/1632457 |
| http://purl.uniprot.org/uniprot/P05121#attribution-B71B329CD240932277FFBBC742BB3B9C | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/1632457 |
| http://purl.uniprot.org/uniprot/P00750#attribution-B71B329CD240932277FFBBC742BB3B9C | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/1632457 |
| http://purl.uniprot.org/uniprot/P04004#attribution-B71B329CD240932277FFBBC742BB3B9C | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/1632457 |