| http://purl.uniprot.org/citations/16326862 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16326862 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16326862 | http://www.w3.org/2000/01/rdf-schema#comment | "Glis2 is a member of the Gli-similar (Glis) subfamily of Krüppel-like zinc finger transcription factors. It functions as an activator and repressor of gene transcription. To identify potential co-activators or co-repressors that mediate these actions of Glis2, we performed yeast two-hybrid analysis using Glis2 as bait. C-terminal binding protein 1 (CtBP1) was identified as one of the proteins that interact with Glis2. This interaction was confirmed by mammalian two-hybrid analysis. CtBP1 did not interact with other members of the Glis subfamily suggesting that this interaction is specific for Glis2. Pulldown analysis with GST-CtBP1 demonstrated that CtBP1 physically interacts with Glis2. Analysis of CtBP1 and Glis2 deletion mutants identified several regions important for this interaction. CtBP1 repressed transcriptional activation induced by Glis2(1-171). Repression by Glis2 appears to involve the recruitment of both CtBP1 and histone deacetylase 3 (HDAC3). Confocal microscopic analysis demonstrated that Glis2 localized to nuclear speckles while in most cells CtBP1 was found diffusely in both cytoplasm and nucleus. However, when CtBP1 and Glis2 were co-expressed, CtBP1 was restricted to nuclear speckles and co-localized with Glis2. Our observations suggest that the co-repressor CtBP1 and HDAC3 are part of transcription silencing complex that mediates the transcriptional repression by Glis2."xsd:string |
| http://purl.uniprot.org/citations/16326862 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/gki985"xsd:string |
| http://purl.uniprot.org/citations/16326862 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/gki985"xsd:string |
| http://purl.uniprot.org/citations/16326862 | http://purl.uniprot.org/core/author | "Kim Y.-S."xsd:string |
| http://purl.uniprot.org/citations/16326862 | http://purl.uniprot.org/core/author | "Kim Y.-S."xsd:string |
| http://purl.uniprot.org/citations/16326862 | http://purl.uniprot.org/core/author | "Kim S.-C."xsd:string |
| http://purl.uniprot.org/citations/16326862 | http://purl.uniprot.org/core/author | "Kim S.-C."xsd:string |
| http://purl.uniprot.org/citations/16326862 | http://purl.uniprot.org/core/author | "Jetten A.M."xsd:string |
| http://purl.uniprot.org/citations/16326862 | http://purl.uniprot.org/core/author | "Jetten A.M."xsd:string |
| http://purl.uniprot.org/citations/16326862 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/16326862 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/16326862 | http://purl.uniprot.org/core/name | "Nucleic Acids Res."xsd:string |
| http://purl.uniprot.org/citations/16326862 | http://purl.uniprot.org/core/name | "Nucleic Acids Res."xsd:string |
| http://purl.uniprot.org/citations/16326862 | http://purl.uniprot.org/core/pages | "6805-6815"xsd:string |
| http://purl.uniprot.org/citations/16326862 | http://purl.uniprot.org/core/pages | "6805-6815"xsd:string |
| http://purl.uniprot.org/citations/16326862 | http://purl.uniprot.org/core/title | "Kruppel-like zinc finger protein Gli-similar 2 (Glis2) represses transcription through interaction with C-terminal binding protein 1 (CtBP1)."xsd:string |
| http://purl.uniprot.org/citations/16326862 | http://purl.uniprot.org/core/title | "Kruppel-like zinc finger protein Gli-similar 2 (Glis2) represses transcription through interaction with C-terminal binding protein 1 (CtBP1)."xsd:string |
| http://purl.uniprot.org/citations/16326862 | http://purl.uniprot.org/core/volume | "33"xsd:string |
| http://purl.uniprot.org/citations/16326862 | http://purl.uniprot.org/core/volume | "33"xsd:string |
| http://purl.uniprot.org/citations/16326862 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16326862 |
| http://purl.uniprot.org/citations/16326862 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16326862 |
| http://purl.uniprot.org/citations/16326862 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/16326862 |
| http://purl.uniprot.org/citations/16326862 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/16326862 |