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http://purl.uniprot.org/citations/16331552http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/16331552http://www.w3.org/2000/01/rdf-schema#comment"

Objective

To investigate the frequency of large fragment aberrations of MSH2 and MLH1 genes from Chinese colorectal cancer (CRC) patients with family history.

Methods

Sixteen exons of MSH2, nineteen exons of MLH1 and seven DNA sequences from the other genes of the samples were screened and checked by multiplex ligation dependent probe amplification (MLPA). First, the methodology was confirmed by testing the positive and negative control samples. Then, 32 CRC or hereditary nonpolyposis colorectal cancer (HNPCC) patients with family history and 20 cases of sporadic CRC were applied to investigate for the large fragment aberrations of MSH2 and MLH1 genes.

Results

The genomic DNA fragment deletions of all positive controls were identified and verified by MLPA. Three cases of 32 familial (hereditary) CRC/HNPCC were detected and identified to be the germline heterozygous deletions of MSH2 gene, of which exons 1-7 were deleted from patient No.3, exon 11 from No.25 and exons 2-6 from No.11. However, no genomic DNA fragment aberration of either MSH2 or MLH1 gene was uncovered from 20 sporadic CRC.

Conclusion

Large DNA fragment aberrations of MSH2 gene was a frequent cause of Chinese HNPCC and CRC patients with family history, and the identification of those aberrations should be included in the regular genetic analysis for CRC/HNPCC patients."xsd:string
http://purl.uniprot.org/citations/16331552http://purl.uniprot.org/core/author"Yuan Y."xsd:string
http://purl.uniprot.org/citations/16331552http://purl.uniprot.org/core/author"Zhu M."xsd:string
http://purl.uniprot.org/citations/16331552http://purl.uniprot.org/core/author"Huang Y.Q."xsd:string
http://purl.uniprot.org/citations/16331552http://purl.uniprot.org/core/author"Zhang Y.Y."xsd:string
http://purl.uniprot.org/citations/16331552http://purl.uniprot.org/core/author"Wang Y.P."xsd:string
http://purl.uniprot.org/citations/16331552http://purl.uniprot.org/core/author"Zhang X.M."xsd:string
http://purl.uniprot.org/citations/16331552http://purl.uniprot.org/core/author"Liu X.R."xsd:string
http://purl.uniprot.org/citations/16331552http://purl.uniprot.org/core/author"Li J.T."xsd:string
http://purl.uniprot.org/citations/16331552http://purl.uniprot.org/core/date"2005"xsd:gYear
http://purl.uniprot.org/citations/16331552http://purl.uniprot.org/core/name"Zhonghua Yi Xue Yi Chuan Xue Za Zhi"xsd:string
http://purl.uniprot.org/citations/16331552http://purl.uniprot.org/core/pages"603-606"xsd:string
http://purl.uniprot.org/citations/16331552http://purl.uniprot.org/core/title"The analysis for identifying large DNA fragment aberrations of MSH2 and MLH1 genes from familial colorectal cancer in China."xsd:string
http://purl.uniprot.org/citations/16331552http://purl.uniprot.org/core/volume"22"xsd:string
http://purl.uniprot.org/citations/16331552http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/16331552
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