| http://purl.uniprot.org/citations/16339725 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16339725 | http://www.w3.org/2000/01/rdf-schema#comment | "Eukaryotic cells require mitochondrial compartments for viability. However, the budding yeast Saccharomyces cerevisiae is able to survive when mitochondrial DNA suffers substantial deletions or is completely absent, so long as a sufficient mitochondrial inner membrane potential is generated. In the absence of functional mitochondrial DNA, and consequently a functional electron transport chain and F(1)F(o)-ATPase, the essential electrical potential is maintained by the electrogenic exchange of ATP(4-) for ADP(3-) through the adenine nucleotide translocator. An essential aspect of this electrogenic process is the conversion of ATP(4-) to ADP(3-) in the mitochondrial matrix, and the nuclear-encoded subunits of F(1)-ATPase are hypothesized to be required for this process in vivo. Deletion of ATP3, the structural gene for the gamma subunit of the F(1)-ATPase, causes yeast to quantitatively lose mitochondrial DNA and grow extremely slowly, presumably by interfering with the generation of an energized inner membrane. A spontaneous suppressor of this slow-growth phenotype was found to convert a conserved glycine to serine in the beta subunit of F(1)-ATPase (atp2-227). This mutation allowed substantial ATP hydrolysis by the F(1)-ATPase even in the absence of the gamma subunit, enabling yeast to generate a twofold greater inner membrane potential in response to ATP compared to mitochondria isolated from yeast lacking the gamma subunit and containing wild-type beta subunits. Analysis of the suppressing mutation by blue native polyacrylamide gel electrophoresis also revealed that the alpha(3)beta(3) heterohexamer can form in the absence of the gamma subunit."xsd:string |
| http://purl.uniprot.org/citations/16339725 | http://purl.org/dc/terms/identifier | "doi:10.1128/ec.4.12.2078-2086.2005"xsd:string |
| http://purl.uniprot.org/citations/16339725 | http://purl.uniprot.org/core/author | "Smith C.P."xsd:string |
| http://purl.uniprot.org/citations/16339725 | http://purl.uniprot.org/core/author | "Thorsness P.E."xsd:string |
| http://purl.uniprot.org/citations/16339725 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/16339725 | http://purl.uniprot.org/core/name | "Eukaryot Cell"xsd:string |
| http://purl.uniprot.org/citations/16339725 | http://purl.uniprot.org/core/pages | "2078-2086"xsd:string |
| http://purl.uniprot.org/citations/16339725 | http://purl.uniprot.org/core/title | "Formation of an energized inner membrane in mitochondria with a gamma-deficient F1-ATPase."xsd:string |
| http://purl.uniprot.org/citations/16339725 | http://purl.uniprot.org/core/volume | "4"xsd:string |
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| http://purl.uniprot.org/uniprot/#_P38077-mappedCitation-16339725 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16339725 |
| http://purl.uniprot.org/uniprot/P00830 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/16339725 |
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| http://purl.uniprot.org/uniprot/P38077 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/16339725 |