| http://purl.uniprot.org/citations/16357217 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16357217 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16357217 | http://www.w3.org/2000/01/rdf-schema#comment | "Two novel yeast splicing factors required for spliceosome disassembly have been identified. Ntr1 and Ntr2 (NineTeen complex-Related proteins) were identified for their weak association with components of the Prp19-associated complex. Unlike other Prp19-associated components, these two proteins were primarily associated with the intron-containing spliceosome during the splicing reaction. Extracts depleted of Ntr1 or Ntr2 exhibited full splicing activity, but accumulated large amounts of lariat-intron in the spliceosome after splicing, indicating that the normal function of the Prp19-associated complex in spliceosome activation was not affected, but spliceosome disassembly was hindered. Immunoprecipitation analysis revealed that Ntr1 and Ntr2 formed a stable complex with DExD/H-box RNA helicase Prp43 in the splicing extract. Ntr1 interacted with Prp43 through the N-terminal G-patch domain, with Ntr2 through a middle region, and with itself through the carboxyl half of the protein. The affinity-purified Ntr1-Ntr2-Prp43 complex could catalyze disassembly of the spliceosome in an ATP-dependent manner, separating U2, U5, U6, NTC (NineTeen Complex), and lariat-intron. This is the first demonstration of physical disassembly of the spliceosome, catalyzed by a complex containing a DExD/H-box RNA helicase and two accessory factors, which might function in targeting the helicase to the correct substrate."xsd:string |
| http://purl.uniprot.org/citations/16357217 | http://purl.org/dc/terms/identifier | "doi:10.1101/gad.1377405"xsd:string |
| http://purl.uniprot.org/citations/16357217 | http://purl.org/dc/terms/identifier | "doi:10.1101/gad.1377405"xsd:string |
| http://purl.uniprot.org/citations/16357217 | http://purl.uniprot.org/core/author | "Huang Y.-H."xsd:string |
| http://purl.uniprot.org/citations/16357217 | http://purl.uniprot.org/core/author | "Huang Y.-H."xsd:string |
| http://purl.uniprot.org/citations/16357217 | http://purl.uniprot.org/core/author | "Lin Y.-C."xsd:string |
| http://purl.uniprot.org/citations/16357217 | http://purl.uniprot.org/core/author | "Lin Y.-C."xsd:string |
| http://purl.uniprot.org/citations/16357217 | http://purl.uniprot.org/core/author | "Cheng S.-C."xsd:string |
| http://purl.uniprot.org/citations/16357217 | http://purl.uniprot.org/core/author | "Cheng S.-C."xsd:string |
| http://purl.uniprot.org/citations/16357217 | http://purl.uniprot.org/core/author | "Fu R.-H."xsd:string |
| http://purl.uniprot.org/citations/16357217 | http://purl.uniprot.org/core/author | "Fu R.-H."xsd:string |
| http://purl.uniprot.org/citations/16357217 | http://purl.uniprot.org/core/author | "Tsai R.-T."xsd:string |
| http://purl.uniprot.org/citations/16357217 | http://purl.uniprot.org/core/author | "Tsai R.-T."xsd:string |
| http://purl.uniprot.org/citations/16357217 | http://purl.uniprot.org/core/author | "Tseng C.-K."xsd:string |
| http://purl.uniprot.org/citations/16357217 | http://purl.uniprot.org/core/author | "Tseng C.-K."xsd:string |
| http://purl.uniprot.org/citations/16357217 | http://purl.uniprot.org/core/author | "Yeh F.-L."xsd:string |
| http://purl.uniprot.org/citations/16357217 | http://purl.uniprot.org/core/author | "Yeh F.-L."xsd:string |
| http://purl.uniprot.org/citations/16357217 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/16357217 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/16357217 | http://purl.uniprot.org/core/name | "Genes Dev."xsd:string |
| http://purl.uniprot.org/citations/16357217 | http://purl.uniprot.org/core/name | "Genes Dev."xsd:string |
| http://purl.uniprot.org/citations/16357217 | http://purl.uniprot.org/core/pages | "2991-3003"xsd:string |
| http://purl.uniprot.org/citations/16357217 | http://purl.uniprot.org/core/pages | "2991-3003"xsd:string |