| http://purl.uniprot.org/citations/16371439 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16371439 | http://www.w3.org/2000/01/rdf-schema#comment | "Pancreatic stellate cells (PSCs) are activated during pancreatitis and promote pancreatic fibrosis by producing and secreting ECMs such as collagen and fibronectin. IL-1beta has been assumed to participate in pancreatic fibrosis by activating PSCs. Activated PSCs secrete various cytokines that regulate PSC function. In this study, we have examined IL-1beta secretion from culture-activated PSCs as well as its regulatory mechanism. RT-PCR and ELISA have demonstrated that PSCs express IL-1beta mRNA and secrete IL-1beta peptide. Inhibition of TGF-beta(1) activity secreted from PSCs by TGF-beta(1)-neutralizing antibody attenuated IL-1beta secretion from PSCs. Exogenous TGF-beta(1) increased IL-1beta expression and secretion by PSCs in a dose-dependent manner. Adenovirus-mediated expression of dominant-negative (dn)Smad2/3 expression reduced both basal and TGF-beta(1)-stimulated IL-1beta expression and secretion by PSCs. Coexpression of Smad3 with dnSmad2/3 restored IL-1beta expression and secretion by PSCs, which were attenuated by dnSmad2/3 expression. In contrast, coexpression of Smad2 with dnSmad2/3 did not alter them. Furthermore, inhibition of IL-1beta activity secreted from PSCs by IL-1beta-neutralizing antibody attenuated TGF-beta(1) secretion from PSCs. Exogenous IL-1beta enhanced TGF-beta(1) expression and secretion by PSCs. IL-1beta activated ERK, and PD-98059, a MEK1 inhibitor, blocked IL-1beta enhancement of TGF-beta(1) expression and secretion by PSCs. We propose that an autocrine loop exists between TGF-beta(1) and IL-1beta in activated PSCs through Smad3- and ERK-dependent pathways."xsd:string |
| http://purl.uniprot.org/citations/16371439 | http://purl.org/dc/terms/identifier | "doi:10.1152/ajpcell.00465.2005"xsd:string |
| http://purl.uniprot.org/citations/16371439 | http://purl.uniprot.org/core/author | "Aoki H."xsd:string |
| http://purl.uniprot.org/citations/16371439 | http://purl.uniprot.org/core/author | "Miyata T."xsd:string |
| http://purl.uniprot.org/citations/16371439 | http://purl.uniprot.org/core/author | "Ohashi A."xsd:string |
| http://purl.uniprot.org/citations/16371439 | http://purl.uniprot.org/core/author | "Sato K."xsd:string |
| http://purl.uniprot.org/citations/16371439 | http://purl.uniprot.org/core/author | "Satoh Y."xsd:string |
| http://purl.uniprot.org/citations/16371439 | http://purl.uniprot.org/core/author | "Yamamoto H."xsd:string |
| http://purl.uniprot.org/citations/16371439 | http://purl.uniprot.org/core/author | "Yasuda H."xsd:string |
| http://purl.uniprot.org/citations/16371439 | http://purl.uniprot.org/core/author | "Ohnishi H."xsd:string |
| http://purl.uniprot.org/citations/16371439 | http://purl.uniprot.org/core/author | "Wada S."xsd:string |
| http://purl.uniprot.org/citations/16371439 | http://purl.uniprot.org/core/author | "Osawa H."xsd:string |
| http://purl.uniprot.org/citations/16371439 | http://purl.uniprot.org/core/author | "Kita H."xsd:string |
| http://purl.uniprot.org/citations/16371439 | http://purl.uniprot.org/core/author | "Mashima H."xsd:string |
| http://purl.uniprot.org/citations/16371439 | http://purl.uniprot.org/core/author | "Tamada K."xsd:string |
| http://purl.uniprot.org/citations/16371439 | http://purl.uniprot.org/core/author | "Hama K."xsd:string |
| http://purl.uniprot.org/citations/16371439 | http://purl.uniprot.org/core/author | "Sugano K."xsd:string |
| http://purl.uniprot.org/citations/16371439 | http://purl.uniprot.org/core/author | "Ishijima T."xsd:string |
| http://purl.uniprot.org/citations/16371439 | http://purl.uniprot.org/core/author | "Hanatsuka K."xsd:string |
| http://purl.uniprot.org/citations/16371439 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16371439 | http://purl.uniprot.org/core/name | "Am J Physiol Cell Physiol"xsd:string |
| http://purl.uniprot.org/citations/16371439 | http://purl.uniprot.org/core/pages | "C1100-8"xsd:string |
| http://purl.uniprot.org/citations/16371439 | http://purl.uniprot.org/core/title | "Autocrine loop between TGF-beta1 and IL-1beta through Smad3- and ERK-dependent pathways in rat pancreatic stellate cells."xsd:string |
| http://purl.uniprot.org/citations/16371439 | http://purl.uniprot.org/core/volume | "290"xsd:string |