| http://purl.uniprot.org/citations/16393980 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16393980 | http://www.w3.org/2000/01/rdf-schema#comment | "We have reported that the TCRzeta mRNA with alternatively spliced 3' UTR (zeta mRNA/as-3'-untranslated region (UTR)) and zeta mRNA lacking exon 7 (zeta mRNA/exon 7-) observed in systemic lupus erythematosus patient T cells can lead to down-regulation of both zeta and TCR/CD3 complexes. To determine whether these T cells expressing decreased zeta exhibit differential transcription patterns, we transfected retrovirus vectors containing wild-type zeta cDNA, zeta cDNA/as-3' UTR, and zeta cDNA/exon 7-into murine T cell hybridoma MA5.8 cells which lack zeta expression to construct the MA5.8 mutants WT, AS3' UTR, and EX7-, respectively. FACS analyses demonstrated reduced cell surface expression of zeta and TCR/CD3 complexes on the AS3' UTR mutant and the EX7-mutant in comparison to that on the WT mutant. Total RNA was collected after stimulating the MA5.8 mutants with anti-CD3 Ab. Reverse-transcribed cDNA was applied to the mouse cDNA microarray containing 8691 genes, and the results were confirmed by real-time PCR. The results showed that 36 genes encoding cytokines and chemokines, including IL-2, IL-15, IL-18, and TGF-beta2, were down-regulated in both the AS3' UTR mutant and the EX7-mutant. Another 16 genes were up-regulated in both, and included genes associated with membranous proteins and cell damage granules, including the genes encoding poliovirus receptor-related 2, syndecan-1, and granzyme A. Increased protein expression of these genes was confirmed by Western blot and FACS analyses. Identification of these responsive genes in T cells in which the zeta and TCR/CD3 complexes were down-regulated may help to better understand the pathogenesis of systemic lupus erythematosus."xsd:string |
| http://purl.uniprot.org/citations/16393980 | http://purl.org/dc/terms/identifier | "doi:10.4049/jimmunol.176.2.949"xsd:string |
| http://purl.uniprot.org/citations/16393980 | http://purl.uniprot.org/core/author | "Abe T."xsd:string |
| http://purl.uniprot.org/citations/16393980 | http://purl.uniprot.org/core/author | "Suzuki K."xsd:string |
| http://purl.uniprot.org/citations/16393980 | http://purl.uniprot.org/core/author | "Yoshimoto K."xsd:string |
| http://purl.uniprot.org/citations/16393980 | http://purl.uniprot.org/core/author | "Takeuchi T."xsd:string |
| http://purl.uniprot.org/citations/16393980 | http://purl.uniprot.org/core/author | "Setoyama Y."xsd:string |
| http://purl.uniprot.org/citations/16393980 | http://purl.uniprot.org/core/author | "Nozaki K."xsd:string |
| http://purl.uniprot.org/citations/16393980 | http://purl.uniprot.org/core/author | "Shiraishi K."xsd:string |
| http://purl.uniprot.org/citations/16393980 | http://purl.uniprot.org/core/author | "Tsuzaka K."xsd:string |
| http://purl.uniprot.org/citations/16393980 | http://purl.uniprot.org/core/author | "Kumazawa C."xsd:string |
| http://purl.uniprot.org/citations/16393980 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16393980 | http://purl.uniprot.org/core/name | "J Immunol"xsd:string |
| http://purl.uniprot.org/citations/16393980 | http://purl.uniprot.org/core/pages | "949-956"xsd:string |
| http://purl.uniprot.org/citations/16393980 | http://purl.uniprot.org/core/title | "DNA microarray gene expression profile of T cells with the splice variants of TCRzeta mRNA observed in systemic lupus erythematosus."xsd:string |
| http://purl.uniprot.org/citations/16393980 | http://purl.uniprot.org/core/volume | "176"xsd:string |
| http://purl.uniprot.org/citations/16393980 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16393980 |
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