| http://purl.uniprot.org/citations/16407231 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16407231 | http://www.w3.org/2000/01/rdf-schema#comment | "Homomeric 5-hydroxytryptamine type 3A receptors (5-HT3ARs) have a single channel conductance (gamma) below the resolution of single channel recording (966 +/-75 fS, estimated by variance analysis). By contrast, heteromeric 5-HT3A/B and nicotinic acetylcholine receptors (nAChRs) have picosiemen range gamma values. In this study, single channel recordings revealed that replacement of cytoplasmic membrane-associated (MA) helix arginine 432 (-4'), 436 (0'), and 440 (4') residues by 5-HT3B (-4'Gln, 0'Asp, and 4'Ala) residues increases gamma to 36.5 +/-1.0 pS. The 0' residue makes the most substantial contribution to gamma of the 5-HT3AR. Replacement of 0'Arg by aspartate, glutamate (alpha7 nAChR subunit MA 0'), or glutamine (beta2 subunit MA 0') increases gamma to the resolvable range (>6 pS). By contrast, replacement of 0'Arg by phenylalanine (alpha4 subunit MA 0') reduced gamma to 416 +/-107 fS. In reciprocal experiments with alpha4beta2 nAChRs (gamma = 31.3 +/-0.8 pS), replacement of MA 0' residues by arginine in alpha4beta2(Q443R) and alpha4(F588R)beta2 reduced gamma slightly. By contrast, the gamma of double mutant alpha4(F588R)beta2(Q443R) was halved. The MA -4' and 4' residues also influenced gamma of 5-HT3ARs. Replacement of nAChR alpha4 or beta2 MA 4' residues by arginine made current density negligible. By contrast, replacement of both -4' residues by arginine produced functional nAChRs with substantially reduced gamma (11.4 +/-0.5 pS). Homology models of the 5-HT3A and alpha4beta2 nAChRs against Torpedo nAChR revealed MA -4', 0', and 4' residues within five intracellular portals. This locus may be a common determinant of ion conduction throughout the Cys loop receptor family."xsd:string |
| http://purl.uniprot.org/citations/16407231 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m513222200"xsd:string |
| http://purl.uniprot.org/citations/16407231 | http://purl.uniprot.org/core/author | "Dunlop J.I."xsd:string |
| http://purl.uniprot.org/citations/16407231 | http://purl.uniprot.org/core/author | "Hales T.G."xsd:string |
| http://purl.uniprot.org/citations/16407231 | http://purl.uniprot.org/core/author | "Kelley S.P."xsd:string |
| http://purl.uniprot.org/citations/16407231 | http://purl.uniprot.org/core/author | "Lambert J.J."xsd:string |
| http://purl.uniprot.org/citations/16407231 | http://purl.uniprot.org/core/author | "Peters J.A."xsd:string |
| http://purl.uniprot.org/citations/16407231 | http://purl.uniprot.org/core/author | "Deeb T.Z."xsd:string |
| http://purl.uniprot.org/citations/16407231 | http://purl.uniprot.org/core/author | "Carland J.E."xsd:string |
| http://purl.uniprot.org/citations/16407231 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16407231 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/16407231 | http://purl.uniprot.org/core/pages | "8062-8071"xsd:string |
| http://purl.uniprot.org/citations/16407231 | http://purl.uniprot.org/core/title | "Common determinants of single channel conductance within the large cytoplasmic loop of 5-hydroxytryptamine type 3 and alpha4beta2 nicotinic acetylcholine receptors."xsd:string |
| http://purl.uniprot.org/citations/16407231 | http://purl.uniprot.org/core/volume | "281"xsd:string |
| http://purl.uniprot.org/citations/16407231 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16407231 |
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