| http://purl.uniprot.org/citations/16407297 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16407297 | http://www.w3.org/2000/01/rdf-schema#comment | "Four and a half LIM protein 1 (FHL1/SLIM1) is highly expressed in skeletal and cardiac muscle; however, the function of FHL1 remains unknown. Yeast two-hybrid screening identified slow type skeletal myosin-binding protein C as an FHL1 binding partner. Myosin-binding protein C is the major myosin-associated protein in striated muscle that enhances the lateral association and stabilization of myosin thick filaments and regulates actomyosin interactions. The interaction between FHL1 and myosin-binding protein C was confirmed using co-immunoprecipitation of recombinant and endogenous proteins. Recombinant FHL2 and FHL3 also bound myosin-binding protein C. FHL1 impaired co-sedimentation of myosin-binding protein C with reconstituted myosin filaments, suggesting FHL1 may compete with myosin for binding to myosin-binding protein C. In intact skeletal muscle and isolated myofibrils, FHL1 localized to the I-band, M-line, and sarcolemma, co-localizing with myosin-binding protein C at the sarcolemma in intact skeletal muscle. Furthermore, in isolated myofibrils FHL1 staining at the M-line appeared to extend partially into the C-zone of the A-band, where it co-localized with myosin-binding protein C. Overexpression of FHL1 in differentiating C2C12 cells induced "sac-like" myotube formation (myosac), associated with impaired Z-line and myosin thick filament assembly. This phenotype was rescued by co-expression of myosin-binding protein C. FHL1 knockdown using RNAi resulted in impaired myosin thick filament formation associated with reduced incorporation of myosin-binding protein C into the sarcomere. This study identified FHL1 as a novel regulator of myosin-binding protein C activity and indicates a role for FHL1 in sarcomere assembly."xsd:string |
| http://purl.uniprot.org/citations/16407297 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m512552200"xsd:string |
| http://purl.uniprot.org/citations/16407297 | http://purl.uniprot.org/core/author | "Brown S."xsd:string |
| http://purl.uniprot.org/citations/16407297 | http://purl.uniprot.org/core/author | "Holdsworth M."xsd:string |
| http://purl.uniprot.org/citations/16407297 | http://purl.uniprot.org/core/author | "Cottle D.L."xsd:string |
| http://purl.uniprot.org/citations/16407297 | http://purl.uniprot.org/core/author | "Hardeman E.C."xsd:string |
| http://purl.uniprot.org/citations/16407297 | http://purl.uniprot.org/core/author | "Mitchell C.A."xsd:string |
| http://purl.uniprot.org/citations/16407297 | http://purl.uniprot.org/core/author | "Robinson P.A."xsd:string |
| http://purl.uniprot.org/citations/16407297 | http://purl.uniprot.org/core/author | "Cowling B.S."xsd:string |
| http://purl.uniprot.org/citations/16407297 | http://purl.uniprot.org/core/author | "McGrath M.J."xsd:string |
| http://purl.uniprot.org/citations/16407297 | http://purl.uniprot.org/core/author | "Coghill I.D."xsd:string |
| http://purl.uniprot.org/citations/16407297 | http://purl.uniprot.org/core/author | "Dyson J.M."xsd:string |
| http://purl.uniprot.org/citations/16407297 | http://purl.uniprot.org/core/author | "Nguyen M.A."xsd:string |
| http://purl.uniprot.org/citations/16407297 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16407297 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/16407297 | http://purl.uniprot.org/core/pages | "7666-7683"xsd:string |
| http://purl.uniprot.org/citations/16407297 | http://purl.uniprot.org/core/title | "Four and a half LIM protein 1 binds myosin-binding protein C and regulates myosin filament formation and sarcomere assembly."xsd:string |
| http://purl.uniprot.org/citations/16407297 | http://purl.uniprot.org/core/volume | "281"xsd:string |
| http://purl.uniprot.org/citations/16407297 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16407297 |
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| http://purl.uniprot.org/uniprot/#_A2AEY2-mappedCitation-16407297 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16407297 |