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http://purl.uniprot.org/citations/16410684http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
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Objective

The aim of the present study was to identify differentially expressed genes that might be associated with the phenotype of superficial and invasive bladder cancer.

Methods

Differential display reverse transcriptase PCR (DDRT-PCR) was used to compare the expression pattern between normal bladder tissue and 4 groups of transitional cell carcinomas of the bladder regarding clinical stage and grade.

Results

We were able to identify 72 different transcripts, of which 57 (79%) showed homology to known genes, 12 (17%) to hypothetical proteins and 3 (4%) to human expressed sequence tags. Among the differentially expressed genes, SFRP1,CEP63 and EIF4G2 were further validated by quantitative RT-PCR in a series of 50 transitional cell carcinomas. Overall, the transcripts of these three genes were shown to be downregulated in the bladder tumors analyzed. In accordance with the DDRT-PCR results, the SFRP1 transcripts were shown to be downregulated in 90% (45/50) of the bladder tumors as compared with the normal bladder tissue. Although EIF4G2 and CEP63 transcripts exhibited three different expression patterns, downregulation was found in about 50% of the cases analyzed. In addition, downregulation of both CEP63 and EIF4G2 gene transcription was associated with invasive tumors.

Conclusion

The use of DDRT-PCR analysis to compare expression patterns among different subgroups of bladder tumors allowed us to identify a significant number of genes implicated in different cellular pathways that, when up- or downregulated, might play a role in the tumorigenic process of the bladder."xsd:string
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http://purl.uniprot.org/citations/16410684http://purl.uniprot.org/core/author"Nagai M.A."xsd:string
http://purl.uniprot.org/citations/16410684http://purl.uniprot.org/core/author"Soares F.A."xsd:string
http://purl.uniprot.org/citations/16410684http://purl.uniprot.org/core/author"Sarkis A.S."xsd:string
http://purl.uniprot.org/citations/16410684http://purl.uniprot.org/core/author"Buim M.E."xsd:string
http://purl.uniprot.org/citations/16410684http://purl.uniprot.org/core/date"2005"xsd:gYear
http://purl.uniprot.org/citations/16410684http://purl.uniprot.org/core/name"Oncology"xsd:string
http://purl.uniprot.org/citations/16410684http://purl.uniprot.org/core/pages"445-454"xsd:string
http://purl.uniprot.org/citations/16410684http://purl.uniprot.org/core/title"The transcripts of SFRP1,CEP63 and EIF4G2 genes are frequently downregulated in transitional cell carcinomas of the bladder."xsd:string
http://purl.uniprot.org/citations/16410684http://purl.uniprot.org/core/volume"69"xsd:string
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