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http://purl.uniprot.org/citations/16446457http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/16446457http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/16446457http://www.w3.org/2000/01/rdf-schema#comment"bZIP-type transcription factors AREBs/ABFs bind an abscisic acid (ABA)-responsive cis-acting element named ABRE and transactivate downstream gene expression in Arabidopsis. Because AREB1 overexpression could not induce downstream gene expression, activation of AREB1 requires ABA-dependent posttranscriptional modification. We confirmed that ABA activated 42-kDa kinase activity, which, in turn, phosphorylated Ser/Thr residues of R-X-X-S/T sites in the conserved regions of AREB1. Amino acid substitutions of R-X-X-S/T sites to Ala suppressed transactivation activity, and multiple substitution of these sites resulted in almost complete suppression of transactivation activity in transient assays. In contrast, substitution of the Ser/Thr residues to Asp resulted in high transactivation activity without exogenous ABA application. A phosphorylated, transcriptionally active form was achieved by substitution of Ser/Thr in all conserved R-X-X-S/T sites to Asp. Transgenic plants overexpressing the phosphorylated active form of AREB1 expressed many ABA-inducible genes, such as RD29B, without ABA treatment. These results indicate that the ABA-dependent multisite phosphorylation of AREB1 regulates its own activation in plants."xsd:string
http://purl.uniprot.org/citations/16446457http://purl.org/dc/terms/identifier"doi:10.1073/pnas.0505667103"xsd:string
http://purl.uniprot.org/citations/16446457http://purl.org/dc/terms/identifier"doi:10.1073/pnas.0505667103"xsd:string
http://purl.uniprot.org/citations/16446457http://purl.uniprot.org/core/author"Fujita Y."xsd:string
http://purl.uniprot.org/citations/16446457http://purl.uniprot.org/core/author"Fujita Y."xsd:string
http://purl.uniprot.org/citations/16446457http://purl.uniprot.org/core/author"Maruyama K."xsd:string
http://purl.uniprot.org/citations/16446457http://purl.uniprot.org/core/author"Maruyama K."xsd:string
http://purl.uniprot.org/citations/16446457http://purl.uniprot.org/core/author"Shinozaki K."xsd:string
http://purl.uniprot.org/citations/16446457http://purl.uniprot.org/core/author"Shinozaki K."xsd:string
http://purl.uniprot.org/citations/16446457http://purl.uniprot.org/core/author"Umezawa T."xsd:string
http://purl.uniprot.org/citations/16446457http://purl.uniprot.org/core/author"Umezawa T."xsd:string
http://purl.uniprot.org/citations/16446457http://purl.uniprot.org/core/author"Yamaguchi-Shinozaki K."xsd:string
http://purl.uniprot.org/citations/16446457http://purl.uniprot.org/core/author"Yamaguchi-Shinozaki K."xsd:string
http://purl.uniprot.org/citations/16446457http://purl.uniprot.org/core/author"Furihata T."xsd:string
http://purl.uniprot.org/citations/16446457http://purl.uniprot.org/core/author"Furihata T."xsd:string
http://purl.uniprot.org/citations/16446457http://purl.uniprot.org/core/author"Yoshida R."xsd:string
http://purl.uniprot.org/citations/16446457http://purl.uniprot.org/core/author"Yoshida R."xsd:string
http://purl.uniprot.org/citations/16446457http://purl.uniprot.org/core/date"2006"xsd:gYear
http://purl.uniprot.org/citations/16446457http://purl.uniprot.org/core/date"2006"xsd:gYear
http://purl.uniprot.org/citations/16446457http://purl.uniprot.org/core/name"Proc. Natl. Acad. Sci. U.S.A."xsd:string
http://purl.uniprot.org/citations/16446457http://purl.uniprot.org/core/name"Proc. Natl. Acad. Sci. U.S.A."xsd:string
http://purl.uniprot.org/citations/16446457http://purl.uniprot.org/core/pages"1988-1993"xsd:string
http://purl.uniprot.org/citations/16446457http://purl.uniprot.org/core/pages"1988-1993"xsd:string