| http://purl.uniprot.org/citations/16464863 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16464863 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16464863 | http://www.w3.org/2000/01/rdf-schema#comment | "We previously reported (Sarfare, S., Ahmad, S. T., Joyce, M. V., Boggess, B., and O'Tousa, J. E. (2005) J. Biol. Chem. 280, 11895-11901) that the Drosophila ninaG gene encodes an oxidoreductase involved in the biosynthesis of the (3S)-3-hydroxyretinal serving as chromophore for Rh1 rhodopsin and that ninaG mutant flies expressing Rh4 as the major opsin accumulate large amounts of a different retinoid. Here, we show that this unknown retinoid is 11-cis-3-hydroxyretinol. Reversed phase high performance liquid chromatography coupled with a photodiode array UV-visible absorbance detector and mass spectrometer revealed a major product eluting at a retention time, t(r), of 3.5 min with a lambda(max) of approximately 324 nm and with a base peak in the mass spectrum at m/z 285. These observations are identical with those of the 3-hydroxyretinol standard. The base peak in the electrospray ionization mass spectrum arises from the loss of a water molecule from the protonated molecule at m/z 303 because of fragmentation in the ion source. These results suggest that 11-cis-3-hydroxyretinol is an intermediate required for chromophore biogenesis in Drosophila. We further show that ninaG mutants fed on retinal as the sole source of vitamin A are able to synthesize 3-hydroxyretinoids. Thus, the NinaG oxidoreductase is not responsible for the initial hydroxylation of the retinal ring but rather acts in a subsequent step in chromophore production. These data are used to review chromophore biosynthesis and propose that NinaG acts in the conversion of (3R)-3-hydroxyretinol to the 3S enantiomer."xsd:string |
| http://purl.uniprot.org/citations/16464863 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m510293200"xsd:string |
| http://purl.uniprot.org/citations/16464863 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m510293200"xsd:string |
| http://purl.uniprot.org/citations/16464863 | http://purl.uniprot.org/core/author | "Ahmad S.T."xsd:string |
| http://purl.uniprot.org/citations/16464863 | http://purl.uniprot.org/core/author | "Ahmad S.T."xsd:string |
| http://purl.uniprot.org/citations/16464863 | http://purl.uniprot.org/core/author | "Boggess B."xsd:string |
| http://purl.uniprot.org/citations/16464863 | http://purl.uniprot.org/core/author | "Boggess B."xsd:string |
| http://purl.uniprot.org/citations/16464863 | http://purl.uniprot.org/core/author | "Joyce M.V."xsd:string |
| http://purl.uniprot.org/citations/16464863 | http://purl.uniprot.org/core/author | "Joyce M.V."xsd:string |
| http://purl.uniprot.org/citations/16464863 | http://purl.uniprot.org/core/author | "O'tousa J.E."xsd:string |
| http://purl.uniprot.org/citations/16464863 | http://purl.uniprot.org/core/author | "O'tousa J.E."xsd:string |
| http://purl.uniprot.org/citations/16464863 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16464863 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16464863 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/16464863 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/16464863 | http://purl.uniprot.org/core/pages | "9205-9209"xsd:string |
| http://purl.uniprot.org/citations/16464863 | http://purl.uniprot.org/core/pages | "9205-9209"xsd:string |
| http://purl.uniprot.org/citations/16464863 | http://purl.uniprot.org/core/title | "The role of Drosophila ninaG oxidoreductase in visual pigment chromophore biogenesis."xsd:string |
| http://purl.uniprot.org/citations/16464863 | http://purl.uniprot.org/core/title | "The role of Drosophila ninaG oxidoreductase in visual pigment chromophore biogenesis."xsd:string |
| http://purl.uniprot.org/citations/16464863 | http://purl.uniprot.org/core/volume | "281"xsd:string |
| http://purl.uniprot.org/citations/16464863 | http://purl.uniprot.org/core/volume | "281"xsd:string |
| http://purl.uniprot.org/citations/16464863 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16464863 |
| http://purl.uniprot.org/citations/16464863 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16464863 |