| http://purl.uniprot.org/citations/16469798 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16469798 | http://www.w3.org/2000/01/rdf-schema#comment | "Chorion is the most abundant site of 11beta-hydroxysteroid dehydrogenase type 1 (11beta-HSD1) expression within intrauterine tissues. It is important to study the regulation of 11beta-HSD1 expression in the chorion in terms of local cortisol production during pregnancy. Using real-time PCR and enzyme activity assay, we found that cortisol (1 mum) and IL-1beta (10 ng/ml) for 24 h significantly increased 11beta-HSD1 mRNA expression and reductase activity in cultured human chorionic trophoblasts. A further significant increase of 11beta-HSD1 mRNA expression and reductase activity was observed with cotreatment of cortisol and IL-1beta. To explore the mechanism of induction, 11beta-HSD1 promoter was cloned into pGL3 plasmid expressing a luciferase reporter gene. By transfecting the constructed vector into WISH cells, an amnion-derived cell line, we found that cortisol (1 microM) or IL-1beta (10 ng/ml) significantly increased reporter gene expression. Likewise, an additional increase in reporter gene expression was observed with cotreatment of cortisol and IL-beta. To explore the physiological significance of 11beta-HSD1 induction in the chorion, we studied the effect of cortisol on cytosolic phospholipase A(2) and cyclooxygenase 2 expression. We found that treatment of chorionic trophoblast cells with cortisol (1 microM) induced both cytosolic phospholipase A(2) and cyclooxygenase 2 mRNA expression. We conclude that cortisol up-regulates 11beta-HSD1 expression through induction of promoter activity, and the effect was enhanced by IL-1beta, suggesting that more biologically active glucocorticoids could be generated in the fetal membranes in the presence of infection, which may consequently feed forward in up-regulation of prostaglandin synthesis."xsd:string |
| http://purl.uniprot.org/citations/16469798 | http://purl.org/dc/terms/identifier | "doi:10.1210/en.2005-1626"xsd:string |
| http://purl.uniprot.org/citations/16469798 | http://purl.uniprot.org/core/author | "Gao L."xsd:string |
| http://purl.uniprot.org/citations/16469798 | http://purl.uniprot.org/core/author | "Li W."xsd:string |
| http://purl.uniprot.org/citations/16469798 | http://purl.uniprot.org/core/author | "Wang Y."xsd:string |
| http://purl.uniprot.org/citations/16469798 | http://purl.uniprot.org/core/author | "Sun K."xsd:string |
| http://purl.uniprot.org/citations/16469798 | http://purl.uniprot.org/core/author | "Myatt L."xsd:string |
| http://purl.uniprot.org/citations/16469798 | http://purl.uniprot.org/core/author | "Duan T."xsd:string |
| http://purl.uniprot.org/citations/16469798 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16469798 | http://purl.uniprot.org/core/name | "Endocrinology"xsd:string |
| http://purl.uniprot.org/citations/16469798 | http://purl.uniprot.org/core/pages | "2490-2495"xsd:string |
| http://purl.uniprot.org/citations/16469798 | http://purl.uniprot.org/core/title | "Enhancement of cortisol-induced 11beta-hydroxysteroid dehydrogenase type 1 expression by interleukin 1beta in cultured human chorionic trophoblast cells."xsd:string |
| http://purl.uniprot.org/citations/16469798 | http://purl.uniprot.org/core/volume | "147"xsd:string |
| http://purl.uniprot.org/citations/16469798 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16469798 |
| http://purl.uniprot.org/citations/16469798 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/16469798 |
| http://purl.uniprot.org/uniprot/#_A0A510GAG5-mappedCitation-16469798 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16469798 |
| http://purl.uniprot.org/uniprot/#_A0AA51YZX7-mappedCitation-16469798 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16469798 |
| http://purl.uniprot.org/uniprot/#_B5BUQ8-mappedCitation-16469798 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16469798 |
| http://purl.uniprot.org/uniprot/#_P28845-mappedCitation-16469798 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16469798 |
| http://purl.uniprot.org/uniprot/#_O43645-mappedCitation-16469798 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16469798 |
| http://purl.uniprot.org/uniprot/#_P01584-mappedCitation-16469798 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16469798 |
| http://purl.uniprot.org/uniprot/#_X5D2L1-mappedCitation-16469798 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16469798 |
| http://purl.uniprot.org/uniprot/A0AA51YZX7 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/16469798 |
| http://purl.uniprot.org/uniprot/B5BUQ8 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/16469798 |