| http://purl.uniprot.org/citations/16476741 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16476741 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16476741 | http://www.w3.org/2000/01/rdf-schema#comment | "Calpain is a Ca2+-regulated cytosolic protease. Mammals have 14 calpain genes, half of which are predominantly expressed in specific organ(s); the rest are expressed ubiquitously. A defect in calpains causes lethality/pathogenicity, indicating their physiological indispensability. nCL-2/calpain-8a was identified as a stomach-specific calpain, whose physiological functions are unclear. To elucidate these, we characterized nCL-2 in detail. Unexpectedly, nCL-2 was localized strictly to the surface mucus cells in the gastric epithelium and the mucus-secreting goblet cells in the duodenum. Yeast two-hybrid screening identified several nCL-2-interacting molecules. Of these, the beta-subunit of coatomer complex (beta-COP) occurs in the stomach pit cells and is proteolyzed by nCL-2 in vitro. Furthermore, beta-COP and nCL-2 co-expressed in COS7 cells co-localized in the Golgi, and Ca2+-ionophore stimulation caused the proteolysis of beta-COP near the linker region, resulting in the dissociation of beta-COP from the Golgi. These results strongly suggest novel functions for nCL-2 that involve the membrane trafficking of mucus cells via interactions with coat protein."xsd:string |
| http://purl.uniprot.org/citations/16476741 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m509244200"xsd:string |
| http://purl.uniprot.org/citations/16476741 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m509244200"xsd:string |
| http://purl.uniprot.org/citations/16476741 | http://purl.uniprot.org/core/author | "Abe K."xsd:string |
| http://purl.uniprot.org/citations/16476741 | http://purl.uniprot.org/core/author | "Abe K."xsd:string |
| http://purl.uniprot.org/citations/16476741 | http://purl.uniprot.org/core/author | "Suzuki K."xsd:string |
| http://purl.uniprot.org/citations/16476741 | http://purl.uniprot.org/core/author | "Suzuki K."xsd:string |
| http://purl.uniprot.org/citations/16476741 | http://purl.uniprot.org/core/author | "Maeda T."xsd:string |
| http://purl.uniprot.org/citations/16476741 | http://purl.uniprot.org/core/author | "Maeda T."xsd:string |
| http://purl.uniprot.org/citations/16476741 | http://purl.uniprot.org/core/author | "Hata S."xsd:string |
| http://purl.uniprot.org/citations/16476741 | http://purl.uniprot.org/core/author | "Hata S."xsd:string |
| http://purl.uniprot.org/citations/16476741 | http://purl.uniprot.org/core/author | "Doi N."xsd:string |
| http://purl.uniprot.org/citations/16476741 | http://purl.uniprot.org/core/author | "Doi N."xsd:string |
| http://purl.uniprot.org/citations/16476741 | http://purl.uniprot.org/core/author | "Sorimachi H."xsd:string |
| http://purl.uniprot.org/citations/16476741 | http://purl.uniprot.org/core/author | "Sorimachi H."xsd:string |
| http://purl.uniprot.org/citations/16476741 | http://purl.uniprot.org/core/author | "Koyama S."xsd:string |
| http://purl.uniprot.org/citations/16476741 | http://purl.uniprot.org/core/author | "Koyama S."xsd:string |
| http://purl.uniprot.org/citations/16476741 | http://purl.uniprot.org/core/author | "Kawahara H."xsd:string |
| http://purl.uniprot.org/citations/16476741 | http://purl.uniprot.org/core/author | "Kawahara H."xsd:string |
| http://purl.uniprot.org/citations/16476741 | http://purl.uniprot.org/core/author | "Toyama-Sorimachi N."xsd:string |
| http://purl.uniprot.org/citations/16476741 | http://purl.uniprot.org/core/author | "Toyama-Sorimachi N."xsd:string |
| http://purl.uniprot.org/citations/16476741 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16476741 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |