| http://purl.uniprot.org/citations/16477523 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16477523 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16477523 | http://www.w3.org/2000/01/rdf-schema#comment | "Using degenerate oligonucleotide primers, we isolated the Caenorhabditis remanei orthologue of the C. elegans sex-determining phosphatase gene fem-2 as well as two other protein phosphatase homologues. Despite the significant sequence divergence between C. elegans and C. remanei FEM-2, we used RNAi-mediated gene knockdown to demonstrate that at least some aspects of male development require FEM-2 function in C. remanei. Consistent with this functional conservation, the conspecific interaction between the FEM-2 and the FEM-3 proteins observed in C. elegans also occurs in C. remanei. To further explore whether the rapid evolution of FEM-2 and FEM-3 affects their molecular interactions, we tested for cross-species interactions between the proteins from C. elegans, C. briggsae, and C. remanei. Although all FEM-2/FEM-3 pairs from a single species interact, only two out of six interspecific pairs bind each other, showing that FEM-2 and FEM-3 are coevolving. Both interspecific interactions involved C. briggsae FEM-3. We constructed chimeric versions of FEM-2 consisting of various combinations of the C. elegans and C. remanei proteins. C. briggsae FEM-3 interacted with all the chimeras, even those that did not interact with either C. elegans or C. remanei FEM-3. We hypothesize that the promiscuity of C. briggsae FEM-3 reflects an increased reliance on evolutionarily constrained regions of FEM-2 for binding. If so, our data support the notion that the coevolution of two interacting proteins sometimes involves a shift in the domains that contribute to binding."xsd:string |
| http://purl.uniprot.org/citations/16477523 | http://purl.org/dc/terms/identifier | "doi:10.1007/s00239-005-0084-5"xsd:string |
| http://purl.uniprot.org/citations/16477523 | http://purl.org/dc/terms/identifier | "doi:10.1007/s00239-005-0084-5"xsd:string |
| http://purl.uniprot.org/citations/16477523 | http://purl.uniprot.org/core/author | "Stothard P."xsd:string |
| http://purl.uniprot.org/citations/16477523 | http://purl.uniprot.org/core/author | "Stothard P."xsd:string |
| http://purl.uniprot.org/citations/16477523 | http://purl.uniprot.org/core/author | "Pilgrim D."xsd:string |
| http://purl.uniprot.org/citations/16477523 | http://purl.uniprot.org/core/author | "Pilgrim D."xsd:string |
| http://purl.uniprot.org/citations/16477523 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16477523 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16477523 | http://purl.uniprot.org/core/name | "J. Mol. Evol."xsd:string |
| http://purl.uniprot.org/citations/16477523 | http://purl.uniprot.org/core/name | "J Mol Evol"xsd:string |
| http://purl.uniprot.org/citations/16477523 | http://purl.uniprot.org/core/pages | "281-291"xsd:string |
| http://purl.uniprot.org/citations/16477523 | http://purl.uniprot.org/core/pages | "281-291"xsd:string |
| http://purl.uniprot.org/citations/16477523 | http://purl.uniprot.org/core/title | "Conspecific and interspecific interactions between the FEM-2 and the FEM-3 sex-determining proteins despite rapid sequence divergence."xsd:string |
| http://purl.uniprot.org/citations/16477523 | http://purl.uniprot.org/core/title | "Conspecific and interspecific interactions between the FEM-2 and the FEM-3 sex-determining proteins despite rapid sequence divergence."xsd:string |
| http://purl.uniprot.org/citations/16477523 | http://purl.uniprot.org/core/volume | "62"xsd:string |
| http://purl.uniprot.org/citations/16477523 | http://purl.uniprot.org/core/volume | "62"xsd:string |
| http://purl.uniprot.org/citations/16477523 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16477523 |
| http://purl.uniprot.org/citations/16477523 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16477523 |
| http://purl.uniprot.org/citations/16477523 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/16477523 |
| http://purl.uniprot.org/citations/16477523 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/16477523 |
| http://purl.uniprot.org/uniprot/Q9U6S2 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/16477523 |
| http://purl.uniprot.org/uniprot/Q8MLZ4 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/16477523 |