| http://purl.uniprot.org/citations/16484680 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16484680 | http://www.w3.org/2000/01/rdf-schema#comment | "Previously, we demonstrated that cysteamine releases endothelin-1 in the rat duodenal mucosa, followed by increased expression of early growth response factor-1 (egr-1). We hypothesized that egr-1 is a key mediator gene in the multifactorial mechanisms of duodenal ulcer development and healing because its protein, transcription factor product Egr-1, regulates the expression of angiogenic growth factors. We wanted to determine the effect of egr-1 antisense oligonucleotide on cysteamine-induced duodenal ulcers as well as on the expression of bFGF, PDGF, and VEGF, of which synthesis is modulated by Egr-1. An antisense oligonucleotide to egr-1 was used to inhibit the synthesis of Egr-1 and to determine its effect on ulcer formation in the rat model of cysteamine-induced duodenal ulceration. Real-time RT-PCR and Western blot analysis were used to assess the expression of Egr-1 mRNA and protein as well as ERK, bFGF, PDGF, and VEGF. The antisense Egr-1 oligonucleotide inhibited the expression of egr-1 mRNA and protein and increased the duodenal ulcer size from 8.1 +/-1.8 mm(2) in controls to 20.7 +/-4.0 mm(2) (P < 0.01). Cysteamine induced phosphorylation of ERK1/2 and enhanced the synthesis of bFGF, PDGF, and VEGF in the preulcerogenic stages of duodenal ulceration, whereas egr-1 antisense oligonucleotide markedly decreased the expression of these growth factors in the duodenal mucosa. We also demonstrated that Egr-1 expression relates to the ulcerogenic effect of cysteamine because these actions were not exerted by the toxic analog ethanolamine. Thus Egr-1 seems to play a critical role in duodenal ulceration because Egr-1 downregulation aggravates experimental duodenal ulcers, most likely through the transcriptional inhibition of bFGF, PDGF, and VEGF synthesis."xsd:string |
| http://purl.uniprot.org/citations/16484680 | http://purl.org/dc/terms/identifier | "doi:10.1152/ajpgi.00078.2005"xsd:string |
| http://purl.uniprot.org/citations/16484680 | http://purl.uniprot.org/core/author | "Chen L."xsd:string |
| http://purl.uniprot.org/citations/16484680 | http://purl.uniprot.org/core/author | "Deng X."xsd:string |
| http://purl.uniprot.org/citations/16484680 | http://purl.uniprot.org/core/author | "Ishikawa H."xsd:string |
| http://purl.uniprot.org/citations/16484680 | http://purl.uniprot.org/core/author | "Szabo S."xsd:string |
| http://purl.uniprot.org/citations/16484680 | http://purl.uniprot.org/core/author | "Jadus M.R."xsd:string |
| http://purl.uniprot.org/citations/16484680 | http://purl.uniprot.org/core/author | "Osapay K."xsd:string |
| http://purl.uniprot.org/citations/16484680 | http://purl.uniprot.org/core/author | "Khomenko T."xsd:string |
| http://purl.uniprot.org/citations/16484680 | http://purl.uniprot.org/core/author | "Sandor Z."xsd:string |
| http://purl.uniprot.org/citations/16484680 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16484680 | http://purl.uniprot.org/core/name | "Am J Physiol Gastrointest Liver Physiol"xsd:string |
| http://purl.uniprot.org/citations/16484680 | http://purl.uniprot.org/core/pages | "G1211-8"xsd:string |
| http://purl.uniprot.org/citations/16484680 | http://purl.uniprot.org/core/title | "Suppression of early growth response factor-1 with egr-1 antisense oligodeoxynucleotide aggravates experimental duodenal ulcers."xsd:string |
| http://purl.uniprot.org/citations/16484680 | http://purl.uniprot.org/core/volume | "290"xsd:string |
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| http://purl.uniprot.org/uniprot/#_P08154-mappedCitation-16484680 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16484680 |
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