| http://purl.uniprot.org/citations/16507368 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16507368 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16507368 | http://www.w3.org/2000/01/rdf-schema#comment | "Tyrosine phosphorylation of cellular proteins induced by extracellular cues serves as a critical mediator in the control of a great variety of cellular processes. Here, we describe an integrated experimental approach including rapid quench methodology and ESI-LC-MS/MS as well as time-resolved ESI-MS to demonstrate that tyrosine autophosphorylation of the catalytic tyrosine kinase domain of FGF-receptor-1 (FGFR1) is mediated by a sequential and precisely ordered reaction. We also demonstrate that the rate of catalysis of two FGFR substrates is enhanced by 50-to 100-fold after autophosphorylation of Y653 in the activation loop, whereas autophosphorylation of the second site in the activation loop (Y654) results in 500-to 1,000-fold increase in the rate of substrate phosphorylation. We propose that FGFR1 is activated by a two-step mechanism mediated by strictly ordered and regulated autophosphorylation, suggesting that distinct phosphorylation states may provide both temporal and spatial resolution to receptor signaling."xsd:string |
| http://purl.uniprot.org/citations/16507368 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.molcel.2006.01.022"xsd:string |
| http://purl.uniprot.org/citations/16507368 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.molcel.2006.01.022"xsd:string |
| http://purl.uniprot.org/citations/16507368 | http://purl.uniprot.org/core/author | "Anderson K.S."xsd:string |
| http://purl.uniprot.org/citations/16507368 | http://purl.uniprot.org/core/author | "Anderson K.S."xsd:string |
| http://purl.uniprot.org/citations/16507368 | http://purl.uniprot.org/core/author | "Furdui C.M."xsd:string |
| http://purl.uniprot.org/citations/16507368 | http://purl.uniprot.org/core/author | "Furdui C.M."xsd:string |
| http://purl.uniprot.org/citations/16507368 | http://purl.uniprot.org/core/author | "Schlessinger J."xsd:string |
| http://purl.uniprot.org/citations/16507368 | http://purl.uniprot.org/core/author | "Schlessinger J."xsd:string |
| http://purl.uniprot.org/citations/16507368 | http://purl.uniprot.org/core/author | "Lew E.D."xsd:string |
| http://purl.uniprot.org/citations/16507368 | http://purl.uniprot.org/core/author | "Lew E.D."xsd:string |
| http://purl.uniprot.org/citations/16507368 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16507368 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16507368 | http://purl.uniprot.org/core/name | "Mol. Cell"xsd:string |
| http://purl.uniprot.org/citations/16507368 | http://purl.uniprot.org/core/name | "Mol. Cell"xsd:string |
| http://purl.uniprot.org/citations/16507368 | http://purl.uniprot.org/core/pages | "711-717"xsd:string |
| http://purl.uniprot.org/citations/16507368 | http://purl.uniprot.org/core/pages | "711-717"xsd:string |
| http://purl.uniprot.org/citations/16507368 | http://purl.uniprot.org/core/title | "Autophosphorylation of FGFR1 kinase is mediated by a sequential and precisely ordered reaction."xsd:string |
| http://purl.uniprot.org/citations/16507368 | http://purl.uniprot.org/core/title | "Autophosphorylation of FGFR1 kinase is mediated by a sequential and precisely ordered reaction."xsd:string |
| http://purl.uniprot.org/citations/16507368 | http://purl.uniprot.org/core/volume | "21"xsd:string |
| http://purl.uniprot.org/citations/16507368 | http://purl.uniprot.org/core/volume | "21"xsd:string |
| http://purl.uniprot.org/citations/16507368 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16507368 |
| http://purl.uniprot.org/citations/16507368 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16507368 |