| http://purl.uniprot.org/citations/16514190 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16514190 | http://www.w3.org/2000/01/rdf-schema#comment | "Cleavage-stage embryos have an absolute requirement for pyruvate and lactate, but as the morula compacts, it switches to glucose as the preferred energy source to fuel glycolysis. Substrates such as glucose, amino acids, and lactate are moved into and out of cells by facilitated diffusion. In the case of lactate and pyruvate, this occurs via H+-monocarboxylate cotransporter (MCT) proteins. To clarify the role of MCT in development, transport characteristics for DL-lactate were examined, as were mRNA expression and protein localisation for MCT1 and MCT3, using confocal laser scanning immunofluorescence in freshly collected and cultured embryos. Blastocysts demonstrated significantly higher affinity for DL-lactate than zygotes (Km 20 +/- 10 vs 87 +/-35 mmol lactate/l; P = 0.03 by linear regression) but was similar for all stages. For embryos derived in vivo and those cultured with glucose, MCT1 mRNA was present throughout preimplantation development, protein immunoreactivity appearing diffuse throughout the cytoplasm with brightest intensity in the outer cortical region of blastomeres. In expanding blastocysts, MCT1 became more prominent in the cytoplasmic cortex of blastomeres, with brightest intensity in the polar trophectoderm. Without glucose, MCT1 mRNA was not expressed, and immunoreactivity dramatically reduced in intensity as morulae died. MCT3 mRNA and immunoreactivity were not detected in early embryos. The differential expression of MCT1 in the presence or absence of glucose demonstrates that it is important in the critical regulation of pH and monocarboxylate transport during preimplantation development, and implies a role for glucose in the control of MCT1, but not MCT3, expression."xsd:string |
| http://purl.uniprot.org/citations/16514190 | http://purl.org/dc/terms/identifier | "doi:10.1530/rep.1.00953"xsd:string |
| http://purl.uniprot.org/citations/16514190 | http://purl.uniprot.org/core/author | "Jansen S."xsd:string |
| http://purl.uniprot.org/citations/16514190 | http://purl.uniprot.org/core/author | "Kaye P.L."xsd:string |
| http://purl.uniprot.org/citations/16514190 | http://purl.uniprot.org/core/author | "Pantaleon M."xsd:string |
| http://purl.uniprot.org/citations/16514190 | http://purl.uniprot.org/core/author | "Esmaeilpour T."xsd:string |
| http://purl.uniprot.org/citations/16514190 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16514190 | http://purl.uniprot.org/core/name | "Reproduction"xsd:string |
| http://purl.uniprot.org/citations/16514190 | http://purl.uniprot.org/core/pages | "469-479"xsd:string |
| http://purl.uniprot.org/citations/16514190 | http://purl.uniprot.org/core/title | "Glucose affects monocarboxylate cotransporter (MCT) 1 expression during mouse preimplantation development."xsd:string |
| http://purl.uniprot.org/citations/16514190 | http://purl.uniprot.org/core/volume | "131"xsd:string |
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