| http://purl.uniprot.org/citations/16516142 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16516142 | http://www.w3.org/2000/01/rdf-schema#comment | "We have previously reported that CPAP (Centrosomal Protein 4.1-Associated Protein) carries a novel microtubule-destabilizing motif that may regulate microtubule dynamics at the centrosome. In this study, we searched for conserved sequence motifs in CPAP and identified two classical 14-3-3 binding sites. The interaction between CPAP and 14-3-3 was demonstrated by both yeast two-hybrid and co-immunoprecipitation experiments. Further analyses revealed that the 14-3-3 binding motif is located within the C-terminal domain of CPAP. Alkaline phosphatase treatment disrupted CPAP binding to 14-3-3, suggesting that phosphorylation is required for the interaction. Mutation of serine 1109 to alanine (S1109A) in the 14-3-3 binding motif completely abolished the association of CPAP with 14-3-3. Taking together, these results imply that phosphorylation of CPAP on serine 1109 is required for 14-3-3 binding. Furthermore, we observed that the interaction between CPAP and 14-3-3 was significantly reduced in mitotic cells, suggesting that 14-3-3 binding to CPAP is regulated during cell cycle progression. In summary, our results show a direct interaction between CPAP and 14-3-3, and this interaction appears to be phosphorylation and cell cycle dependent."xsd:string |
| http://purl.uniprot.org/citations/16516142 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbrc.2006.02.089"xsd:string |
| http://purl.uniprot.org/citations/16516142 | http://purl.uniprot.org/core/author | "Chen C.Y."xsd:string |
| http://purl.uniprot.org/citations/16516142 | http://purl.uniprot.org/core/author | "Olayioye M.A."xsd:string |
| http://purl.uniprot.org/citations/16516142 | http://purl.uniprot.org/core/author | "Tang T.K."xsd:string |
| http://purl.uniprot.org/citations/16516142 | http://purl.uniprot.org/core/author | "Lindeman G.J."xsd:string |
| http://purl.uniprot.org/citations/16516142 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16516142 | http://purl.uniprot.org/core/name | "Biochem Biophys Res Commun"xsd:string |
| http://purl.uniprot.org/citations/16516142 | http://purl.uniprot.org/core/pages | "1203-1210"xsd:string |
| http://purl.uniprot.org/citations/16516142 | http://purl.uniprot.org/core/title | "CPAP interacts with 14-3-3 in a cell cycle-dependent manner."xsd:string |
| http://purl.uniprot.org/citations/16516142 | http://purl.uniprot.org/core/volume | "342"xsd:string |
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