| http://purl.uniprot.org/citations/1652440 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1652440 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1652440 | http://www.w3.org/2000/01/rdf-schema#comment | "Immunocytochemical investigations have previously shown that antibodies specific for mammal connexins labeled in situ rat and mouse brain gap junctions. However brain gap-junction proteins have neither been identified with certainty, nor purified. By immunoblotting, anti-peptide antibodies directed against rat heart connexin 43 (CX43) detect a major protein of 41 kDa in rat brain homogenates. The specificity of these antibodies made it possible to establish an affinity-chromatography purification procedure of the 41-kDa protein. Purified antibodies specific for the sequence SAEQNRMGQ (residues 314-322) of rat heart CX43 were covalently bound to a protein-A-Sepharose-CL-4B matrix. Rat brain homogenates were recycled through the immunomatrix and the material specifically bound to the matrix was then competitively eluted with the peptide SAEQNRMGQY. Analysis by SDS/PAGE of eluates demonstrated that they contain a 41-kDa protein associated with low amounts of high-molecular-mass proteins. By immunoblotting, these proteins were shown to be specifically recognized by antibodies directed against residues 5-17, 55-56, and 314-322 of rat heart CX43. The NH2-terminal partial sequence for the 41-kDa protein was determined by microsequencing and shown to be similar to alpha 1 connexins. This is the first successful purification of a junctional protein from brain tissue and provides direct evidence that the 41-kDa protein is a CX43 gene product."xsd:string |
| http://purl.uniprot.org/citations/1652440 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1432-1033.1991.tb21075.x"xsd:string |
| http://purl.uniprot.org/citations/1652440 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1432-1033.1991.tb21075.x"xsd:string |
| http://purl.uniprot.org/citations/1652440 | http://purl.uniprot.org/core/author | "Dupont E."xsd:string |
| http://purl.uniprot.org/citations/1652440 | http://purl.uniprot.org/core/author | "Dupont E."xsd:string |
| http://purl.uniprot.org/citations/1652440 | http://purl.uniprot.org/core/author | "Briand J.-C."xsd:string |
| http://purl.uniprot.org/citations/1652440 | http://purl.uniprot.org/core/author | "Briand J.-C."xsd:string |
| http://purl.uniprot.org/citations/1652440 | http://purl.uniprot.org/core/author | "Fromaget C."xsd:string |
| http://purl.uniprot.org/citations/1652440 | http://purl.uniprot.org/core/author | "Fromaget C."xsd:string |
| http://purl.uniprot.org/citations/1652440 | http://purl.uniprot.org/core/author | "Gros D."xsd:string |
| http://purl.uniprot.org/citations/1652440 | http://purl.uniprot.org/core/author | "Gros D."xsd:string |
| http://purl.uniprot.org/citations/1652440 | http://purl.uniprot.org/core/author | "el Aoumari A."xsd:string |
| http://purl.uniprot.org/citations/1652440 | http://purl.uniprot.org/core/author | "el Aoumari A."xsd:string |
| http://purl.uniprot.org/citations/1652440 | http://purl.uniprot.org/core/date | "1991"xsd:gYear |
| http://purl.uniprot.org/citations/1652440 | http://purl.uniprot.org/core/date | "1991"xsd:gYear |
| http://purl.uniprot.org/citations/1652440 | http://purl.uniprot.org/core/name | "Eur. J. Biochem."xsd:string |
| http://purl.uniprot.org/citations/1652440 | http://purl.uniprot.org/core/name | "Eur. J. Biochem."xsd:string |
| http://purl.uniprot.org/citations/1652440 | http://purl.uniprot.org/core/pages | "263-270"xsd:string |
| http://purl.uniprot.org/citations/1652440 | http://purl.uniprot.org/core/pages | "263-270"xsd:string |
| http://purl.uniprot.org/citations/1652440 | http://purl.uniprot.org/core/title | "Affinity purification of a rat-brain junctional protein, connexin 43."xsd:string |
| http://purl.uniprot.org/citations/1652440 | http://purl.uniprot.org/core/title | "Affinity purification of a rat-brain junctional protein, connexin 43."xsd:string |
| http://purl.uniprot.org/citations/1652440 | http://purl.uniprot.org/core/volume | "200"xsd:string |
| http://purl.uniprot.org/citations/1652440 | http://purl.uniprot.org/core/volume | "200"xsd:string |