| http://purl.uniprot.org/citations/16537372 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16537372 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16537372 | http://www.w3.org/2000/01/rdf-schema#comment | "The covalent attachment of heme cofactors to the apo-polypeptides via thioether bonds is unique to the maturation of c-type cytochromes. A number of thiol-disulfide oxidoreductases prepare the apocytochrome for heme insertion in system I and II cytochrome c maturation. Although most thiol-disulfide oxidoreductases are nonspecific, the less common, specific thiol-disulfide oxidoreductases may be key to directing the usage of electrons. Here we demonstrate that unlike other thiol-disulfide oxidoreductases, the protein responsible for reducing oxidized apocytochrome c in Bacillus subtilis, ResA, is specific for cytochrome c550 and utilizes alternate conformations to recognize redox partners. We report solution NMR evidence that ResA undergoes a redox-dependent conformational change between oxidation states, as well as data showing that ResA utilizes a surface cavity present only in the reduced state to recognize a peptide derived from cytochrome c550. Finally, we confirm that ResA is a specific thiol-disulfide oxidoreductase by comparing its reactivity to our mimetic peptide with its reactivity to oxidized glutathione, a nonspecific substrate. This study biochemically demonstrates the specificity of this thiol-disulfide oxidoreductase and enables us to outline a structural mechanism of regulating the usage of electrons in a thiol-disulfide oxidoreductase system."xsd:string |
| http://purl.uniprot.org/citations/16537372 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.0600552103"xsd:string |
| http://purl.uniprot.org/citations/16537372 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.0600552103"xsd:string |
| http://purl.uniprot.org/citations/16537372 | http://purl.uniprot.org/core/author | "Colbert C.L."xsd:string |
| http://purl.uniprot.org/citations/16537372 | http://purl.uniprot.org/core/author | "Colbert C.L."xsd:string |
| http://purl.uniprot.org/citations/16537372 | http://purl.uniprot.org/core/author | "Wu Q."xsd:string |
| http://purl.uniprot.org/citations/16537372 | http://purl.uniprot.org/core/author | "Wu Q."xsd:string |
| http://purl.uniprot.org/citations/16537372 | http://purl.uniprot.org/core/author | "Deisenhofer J."xsd:string |
| http://purl.uniprot.org/citations/16537372 | http://purl.uniprot.org/core/author | "Deisenhofer J."xsd:string |
| http://purl.uniprot.org/citations/16537372 | http://purl.uniprot.org/core/author | "Gardner K.H."xsd:string |
| http://purl.uniprot.org/citations/16537372 | http://purl.uniprot.org/core/author | "Gardner K.H."xsd:string |
| http://purl.uniprot.org/citations/16537372 | http://purl.uniprot.org/core/author | "Erbel P.J.A."xsd:string |
| http://purl.uniprot.org/citations/16537372 | http://purl.uniprot.org/core/author | "Erbel P.J.A."xsd:string |
| http://purl.uniprot.org/citations/16537372 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16537372 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16537372 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/16537372 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/16537372 | http://purl.uniprot.org/core/pages | "4410-4415"xsd:string |
| http://purl.uniprot.org/citations/16537372 | http://purl.uniprot.org/core/pages | "4410-4415"xsd:string |
| http://purl.uniprot.org/citations/16537372 | http://purl.uniprot.org/core/title | "Mechanism of substrate specificity in Bacillus subtilis ResA, a thioredoxin-like protein involved in cytochrome c maturation."xsd:string |
| http://purl.uniprot.org/citations/16537372 | http://purl.uniprot.org/core/title | "Mechanism of substrate specificity in Bacillus subtilis ResA, a thioredoxin-like protein involved in cytochrome c maturation."xsd:string |
| http://purl.uniprot.org/citations/16537372 | http://purl.uniprot.org/core/volume | "103"xsd:string |
| http://purl.uniprot.org/citations/16537372 | http://purl.uniprot.org/core/volume | "103"xsd:string |