| http://purl.uniprot.org/citations/1654338 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1654338 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1654338 | http://www.w3.org/2000/01/rdf-schema#comment | "Human umbilical vein endothelial (HUVE) cells have been previously reported to express the genes for the A and B chains of PDGF and to secrete PDGF-related factors into culture media. Antihuman PDGF IgG affinity chromatography was used to purify PDGF-related activity from HUVE cell-conditioned media. Immunoblot analysis of the affinity-purified proteins with anti-PDGF IgG and antibodies specific for the A or B chain peptides of PDGF combined with chemotactic and mitogenic assays revealed that the major PDGF immunorelated molecule secreted by HUVE cells is a monomer of approximately 36-38 kD and that less than 10% of the purified biologically active molecules are PDGF A or B chain peptides. Screening of an HUVE cell cDNA library in the expression vector lambda gtl 1 with the anti-PDGF antibody resulted in the cloning and sequencing of a cDNA with an open reading frame encoding a 38-kD cysteine-rich secreted protein which we show to be the major PDGF-related mitogen secreted by human vascular endothelial cells. The protein has a 45% overall homology to the translation product of the v-src-induced CEF-10 mRNA from chick embryo fibroblasts. We have termed this new mitogen connective tissue growth factor."xsd:string |
| http://purl.uniprot.org/citations/1654338 | http://purl.org/dc/terms/identifier | "doi:10.1083/jcb.114.6.1285"xsd:string |
| http://purl.uniprot.org/citations/1654338 | http://purl.org/dc/terms/identifier | "doi:10.1083/jcb.114.6.1285"xsd:string |
| http://purl.uniprot.org/citations/1654338 | http://purl.uniprot.org/core/author | "Igarashi A."xsd:string |
| http://purl.uniprot.org/citations/1654338 | http://purl.uniprot.org/core/author | "Igarashi A."xsd:string |
| http://purl.uniprot.org/citations/1654338 | http://purl.uniprot.org/core/author | "Bradham D.M."xsd:string |
| http://purl.uniprot.org/citations/1654338 | http://purl.uniprot.org/core/author | "Bradham D.M."xsd:string |
| http://purl.uniprot.org/citations/1654338 | http://purl.uniprot.org/core/author | "Grotendorst G.R."xsd:string |
| http://purl.uniprot.org/citations/1654338 | http://purl.uniprot.org/core/author | "Grotendorst G.R."xsd:string |
| http://purl.uniprot.org/citations/1654338 | http://purl.uniprot.org/core/author | "Potter R.L."xsd:string |
| http://purl.uniprot.org/citations/1654338 | http://purl.uniprot.org/core/author | "Potter R.L."xsd:string |
| http://purl.uniprot.org/citations/1654338 | http://purl.uniprot.org/core/date | "1991"xsd:gYear |
| http://purl.uniprot.org/citations/1654338 | http://purl.uniprot.org/core/date | "1991"xsd:gYear |
| http://purl.uniprot.org/citations/1654338 | http://purl.uniprot.org/core/name | "J. Cell Biol."xsd:string |
| http://purl.uniprot.org/citations/1654338 | http://purl.uniprot.org/core/name | "J. Cell Biol."xsd:string |
| http://purl.uniprot.org/citations/1654338 | http://purl.uniprot.org/core/pages | "1285-1294"xsd:string |
| http://purl.uniprot.org/citations/1654338 | http://purl.uniprot.org/core/pages | "1285-1294"xsd:string |
| http://purl.uniprot.org/citations/1654338 | http://purl.uniprot.org/core/title | "Connective tissue growth factor: a cysteine-rich mitogen secreted by human vascular endothelial cells is related to the SRC-induced immediate early gene product CEF-10."xsd:string |
| http://purl.uniprot.org/citations/1654338 | http://purl.uniprot.org/core/title | "Connective tissue growth factor: a cysteine-rich mitogen secreted by human vascular endothelial cells is related to the SRC-induced immediate early gene product CEF-10."xsd:string |
| http://purl.uniprot.org/citations/1654338 | http://purl.uniprot.org/core/volume | "114"xsd:string |
| http://purl.uniprot.org/citations/1654338 | http://purl.uniprot.org/core/volume | "114"xsd:string |
| http://purl.uniprot.org/citations/1654338 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/1654338 |
| http://purl.uniprot.org/citations/1654338 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/1654338 |