| http://purl.uniprot.org/citations/1654894 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1654894 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1654894 | http://www.w3.org/2000/01/rdf-schema#comment | "A human hippocampus cDNA library in lambda ZAP II was screened by hybridization with a rat brain inositol 1,4,5-trisphosphate (InsP3) 3-kinase cDNA. Two clones (hh6 and hh3) were isolated and sequenced. The insert of clone hh6 was shown to correspond to the 3' end of the coding sequence of 50,000-Mr InsP3 3-kinase (referred to as 3-kinase-A). Sequencing of the clone hh3 insert yielded an open reading frame encoding a 472-amino acid protein with a calculated Mr of 53,451 (referred to as 3-kinase-B). The C-terminal part of 3-kinase-B (residues 187-462) was 68% identical with 3-kinase-A in amino acid sequence. The cDNA of clone hh3 was rescued as a Bluescript plasmid and expressed in Escherichia coli as a beta-galactosidase fusion product. It showed InsP3 3-kinase activity that was stimulated in the presence of Ca2+/calmodulin (more than 7-fold in a crude bacterial lysate from expressed plasmid). Regeneration of InsP3 3-kinase activity after SDS/PAGE identified a major polypeptide (Mr 60,000-65,000). The Km for InsP3 of expressed 3-kinase-B was 1.6 microM. These data provide molecular evidence for the existence of InsP3 3-kinase isoenzymes."xsd:string |
| http://purl.uniprot.org/citations/1654894 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj2780883"xsd:string |
| http://purl.uniprot.org/citations/1654894 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj2780883"xsd:string |
| http://purl.uniprot.org/citations/1654894 | http://purl.uniprot.org/core/author | "Dumont J.E."xsd:string |
| http://purl.uniprot.org/citations/1654894 | http://purl.uniprot.org/core/author | "Dumont J.E."xsd:string |
| http://purl.uniprot.org/citations/1654894 | http://purl.uniprot.org/core/author | "Erneux C."xsd:string |
| http://purl.uniprot.org/citations/1654894 | http://purl.uniprot.org/core/author | "Erneux C."xsd:string |
| http://purl.uniprot.org/citations/1654894 | http://purl.uniprot.org/core/author | "Perret J."xsd:string |
| http://purl.uniprot.org/citations/1654894 | http://purl.uniprot.org/core/author | "Perret J."xsd:string |
| http://purl.uniprot.org/citations/1654894 | http://purl.uniprot.org/core/author | "Takazawa K."xsd:string |
| http://purl.uniprot.org/citations/1654894 | http://purl.uniprot.org/core/author | "Takazawa K."xsd:string |
| http://purl.uniprot.org/citations/1654894 | http://purl.uniprot.org/core/date | "1991"xsd:gYear |
| http://purl.uniprot.org/citations/1654894 | http://purl.uniprot.org/core/date | "1991"xsd:gYear |
| http://purl.uniprot.org/citations/1654894 | http://purl.uniprot.org/core/name | "Biochem. J."xsd:string |
| http://purl.uniprot.org/citations/1654894 | http://purl.uniprot.org/core/name | "Biochem. J."xsd:string |
| http://purl.uniprot.org/citations/1654894 | http://purl.uniprot.org/core/pages | "883-886"xsd:string |
| http://purl.uniprot.org/citations/1654894 | http://purl.uniprot.org/core/pages | "883-886"xsd:string |
| http://purl.uniprot.org/citations/1654894 | http://purl.uniprot.org/core/title | "Molecular cloning and expression of a new putative inositol 1,4,5-trisphosphate 3-kinase isoenzyme."xsd:string |
| http://purl.uniprot.org/citations/1654894 | http://purl.uniprot.org/core/title | "Molecular cloning and expression of a new putative inositol 1,4,5-trisphosphate 3-kinase isoenzyme."xsd:string |
| http://purl.uniprot.org/citations/1654894 | http://purl.uniprot.org/core/volume | "278"xsd:string |
| http://purl.uniprot.org/citations/1654894 | http://purl.uniprot.org/core/volume | "278"xsd:string |
| http://purl.uniprot.org/citations/1654894 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/1654894 |
| http://purl.uniprot.org/citations/1654894 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/1654894 |