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http://purl.uniprot.org/citations/16565389http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
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Purpose

The peripheral tolerance that arises after injection of antigen into the anterior chamber (anterior chamber-associated immune deviation; ACAID) is associated in part with CD8+ T cells that suppress the expression of Th1 and Th2 immunity. The purpose of these studies was to determine the genes and molecules that are critical for CD8+ T regulatory cell (T reg) functions in ACAID.

Methods

Ovalbumin (OVA)-specific CD8+ T cells from T-cell receptor (TCR) transgenic OT-1 mice acquire efferent regulatory properties similar to in vivo-generated CD8+ T regs after stimulation with OVA-pulsed TGF-beta2-treated APCs. Changes in the genetic program associated with acquisition of efferent regulatory function in OT-1 CD8+ T cells in vitro were determined by DNA microarray analyses and confirmed by RT-PCR analyses and biological assays.

Results

T regulatory OT-1 T cells acquired a novel transcriptional pattern indicative of their function. Genes for molecules associated with TGF-beta function, resistance to TCR-triggered apoptosis, and localization of cells to antigen deposition in peripheral tissues were upregulated, and genes related to cytolytic function were downregulated. Further study showed that CD103, a cell-adhesion molecule that binds E-cadherin, was highly upregulated in in vivo-generated ACAID T regs and was necessary for their suppression of T-cell activation in vitro.

Conclusions

OT-1 CD8 T cells modulated in vitro by exposure to antigen-pulsed, TGF-beta2-treated APCs expressed genes related to immune suppression. Thus, the necessity for CD103 emerges in the efferent CD8+ T-cell regulatory mechanisms in eye-derived tolerance."xsd:string
http://purl.uniprot.org/citations/16565389http://purl.org/dc/terms/identifier"doi:10.1167/iovs.04-1454"xsd:string
http://purl.uniprot.org/citations/16565389http://purl.uniprot.org/core/author"Sasaki S."xsd:string
http://purl.uniprot.org/citations/16565389http://purl.uniprot.org/core/author"Stein-Streilein J."xsd:string
http://purl.uniprot.org/citations/16565389http://purl.uniprot.org/core/author"Keino H."xsd:string
http://purl.uniprot.org/citations/16565389http://purl.uniprot.org/core/author"Streilein J.W."xsd:string
http://purl.uniprot.org/citations/16565389http://purl.uniprot.org/core/author"Masli S."xsd:string
http://purl.uniprot.org/citations/16565389http://purl.uniprot.org/core/date"2006"xsd:gYear
http://purl.uniprot.org/citations/16565389http://purl.uniprot.org/core/name"Invest Ophthalmol Vis Sci"xsd:string
http://purl.uniprot.org/citations/16565389http://purl.uniprot.org/core/pages"1533-1542"xsd:string
http://purl.uniprot.org/citations/16565389http://purl.uniprot.org/core/title"CD8+ T regulatory cells use a novel genetic program that includes CD103 to suppress Th1 immunity in eye-derived tolerance."xsd:string
http://purl.uniprot.org/citations/16565389http://purl.uniprot.org/core/volume"47"xsd:string
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