| http://purl.uniprot.org/citations/16635983 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16635983 | http://www.w3.org/2000/01/rdf-schema#comment | "Typical mass spectrometry-based protein lists from purified fractions are confounded by the absence of tools for evaluating contaminants. In this report, we compare the results of a standard survey experiment using an ion trap mass spectrometer with those obtained using dual isotope labeling and a Q-TOF mass spectrometer to quantify the degree of enrichment of proteins in purified subcellular fractions of Arabidopsis plasma membrane. Incorporation of a stable isotope, either H(2)(18)O or H(2)(16)O, during trypsinization allowed relative quantification of the degree of enrichment of proteins within membranes after phase partitioning with polyethylene glycol/dextran mixtures. The ratios allowed the quantification of 174 membrane-associated proteins with 70 showing plasma membrane enrichment equal to or greater than ATP-dependent proton pumps, canonical plasma membrane proteins. Enriched proteins included several hallmark plasma membrane proteins, such as H(+)-ATPases, aquaporins, receptor-like kinases, and various transporters, as well as a number of proteins with unknown functions. Most importantly, a comparison of the datasets from a sequencing "survey" analysis using the ion trap mass spectrometer with that from the quantitative dual isotope labeling ratio method indicates that as many as one-fourth of the putative survey identifications are biological contaminants rather than bona fide plasma membrane proteins."xsd:string |
| http://purl.uniprot.org/citations/16635983 | http://purl.org/dc/terms/identifier | "doi:10.1074/mcp.m500414-mcp200"xsd:string |
| http://purl.uniprot.org/citations/16635983 | http://purl.uniprot.org/core/author | "Sussman M.R."xsd:string |
| http://purl.uniprot.org/citations/16635983 | http://purl.uniprot.org/core/author | "Nelson C.J."xsd:string |
| http://purl.uniprot.org/citations/16635983 | http://purl.uniprot.org/core/author | "Hegeman A.D."xsd:string |
| http://purl.uniprot.org/citations/16635983 | http://purl.uniprot.org/core/author | "Harms A.C."xsd:string |
| http://purl.uniprot.org/citations/16635983 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16635983 | http://purl.uniprot.org/core/name | "Mol Cell Proteomics"xsd:string |
| http://purl.uniprot.org/citations/16635983 | http://purl.uniprot.org/core/pages | "1382-1395"xsd:string |
| http://purl.uniprot.org/citations/16635983 | http://purl.uniprot.org/core/title | "A quantitative analysis of Arabidopsis plasma membrane using trypsin-catalyzed (18)O labeling."xsd:string |
| http://purl.uniprot.org/citations/16635983 | http://purl.uniprot.org/core/volume | "5"xsd:string |
| http://purl.uniprot.org/citations/16635983 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16635983 |
| http://purl.uniprot.org/citations/16635983 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/16635983 |
| http://purl.uniprot.org/uniprot/#_A0A178V207-mappedCitation-16635983 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16635983 |
| http://purl.uniprot.org/uniprot/#_F4JUC2-mappedCitation-16635983 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16635983 |
| http://purl.uniprot.org/uniprot/#_F4JUT8-mappedCitation-16635983 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16635983 |
| http://purl.uniprot.org/uniprot/#_F4JUT9-mappedCitation-16635983 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16635983 |
| http://purl.uniprot.org/uniprot/#_F4K0D0-mappedCitation-16635983 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16635983 |
| http://purl.uniprot.org/uniprot/#_F4K3I6-mappedCitation-16635983 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16635983 |
| http://purl.uniprot.org/uniprot/#_F4K3R8-mappedCitation-16635983 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16635983 |
| http://purl.uniprot.org/uniprot/#_F4K9K2-mappedCitation-16635983 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16635983 |
| http://purl.uniprot.org/uniprot/#_F4K9K4-mappedCitation-16635983 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16635983 |
| http://purl.uniprot.org/uniprot/#_A0A2H1ZE94-mappedCitation-16635983 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16635983 |
| http://purl.uniprot.org/uniprot/#_A0A1I9LQD4-mappedCitation-16635983 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16635983 |