| http://purl.uniprot.org/citations/16644729 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16644729 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16644729 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/16644729 | http://www.w3.org/2000/01/rdf-schema#comment | "Dictyostelium discoideum cells possess multiple cyclic nucleotide phosphodiesterases that belong either to class I enzymes that are present in all eukaryotes or to the rare beta-lactamase class II. We describe here the identification and characterization of DdPDE4, the third class I enzyme of Dictyostelium. The deduced amino acid sequence predicts that DdPDE4 has a leader sequence, two transmembrane segments, and an extracellular catalytic domain that exhibits a high degree of homology with human cAMP-specific PDE8. Expression of the catalytic domain of DdPDE4 shows that the enzyme is a cAMP-specific phosphodiesterase with a K(m) of 10 microm; cGMP is hydrolyzed at least 100-fold more slowly. The full-length protein is shown to be membrane-bound with catalytic activity exposed to the extracellular medium. Northern blots and activity measurements reveal that expression of DdPDE4 is low during single cell stages and increases at 9 h of starvation, corresponding with mound stage. A function during multicellular development is confirmed by the phenotype of ddpde4(-) knock-out strains, showing normal aggregation but impaired development from the mound stage on. These results demonstrate that DdPDE4 is a unique membrane-bound phosphodiesterase with an extracellular catalytic domain regulating intercellular cAMP during multicellular development."xsd:string |
| http://purl.uniprot.org/citations/16644729 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m600040200"xsd:string |
| http://purl.uniprot.org/citations/16644729 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m600040200"xsd:string |
| http://purl.uniprot.org/citations/16644729 | http://purl.uniprot.org/core/author | "Van Haastert P.J.M."xsd:string |
| http://purl.uniprot.org/citations/16644729 | http://purl.uniprot.org/core/author | "Van Haastert P.J.M."xsd:string |
| http://purl.uniprot.org/citations/16644729 | http://purl.uniprot.org/core/author | "Kortholt A."xsd:string |
| http://purl.uniprot.org/citations/16644729 | http://purl.uniprot.org/core/author | "Kortholt A."xsd:string |
| http://purl.uniprot.org/citations/16644729 | http://purl.uniprot.org/core/author | "Snippe H."xsd:string |
| http://purl.uniprot.org/citations/16644729 | http://purl.uniprot.org/core/author | "Snippe H."xsd:string |
| http://purl.uniprot.org/citations/16644729 | http://purl.uniprot.org/core/author | "Bader S."xsd:string |
| http://purl.uniprot.org/citations/16644729 | http://purl.uniprot.org/core/author | "Bader S."xsd:string |
| http://purl.uniprot.org/citations/16644729 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16644729 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16644729 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/16644729 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/16644729 | http://purl.uniprot.org/core/pages | "20018-20026"xsd:string |
| http://purl.uniprot.org/citations/16644729 | http://purl.uniprot.org/core/pages | "20018-20026"xsd:string |
| http://purl.uniprot.org/citations/16644729 | http://purl.uniprot.org/core/title | "DdPDE4, a novel cAMP-specific phosphodiesterase at the surface of dictyostelium cells."xsd:string |
| http://purl.uniprot.org/citations/16644729 | http://purl.uniprot.org/core/title | "DdPDE4, a novel cAMP-specific phosphodiesterase at the surface of dictyostelium cells."xsd:string |
| http://purl.uniprot.org/citations/16644729 | http://purl.uniprot.org/core/volume | "281"xsd:string |
| http://purl.uniprot.org/citations/16644729 | http://purl.uniprot.org/core/volume | "281"xsd:string |
| http://purl.uniprot.org/citations/16644729 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16644729 |