| http://purl.uniprot.org/citations/1668278 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1668278 | http://www.w3.org/2000/01/rdf-schema#comment | "Previously, 5' deletion analysis revealed three important upstream regions within the regulatory region of the cAMP-induced, prespore gene SP60 of D. discoidium, each of which contains a CA-rich sequence element (CAE: consensus CACACAYYYCACACAAA/T). In this study, we have made site-directed mutations within these CAEs and examined their effect on reporter gene activity (luciferase or lacZ). Point mutations within or deletion of the distal CAE (CAE-1), middle CAE (CAE-2) or proximal CAE (CAE-3) result in substantial decreases in promoter activity at 18 h of development or in response to cAMP. lacZ fusions made with the CAE mutant promoters produced novel beta-gal staining patterns that suggest the presence of one or more morphogen gradients within the prespore zone of the slug and indicate that the CAEs are also important in regulating the spatial patterning of SP60 expression in the multicellular aggregate. Gel mobility shift assays were used to identify activities from crude nuclear extracts that bind oligonucleotides containing the CAEs. One of the binding activities is not observed in extracts from vegetative cells or cells in early development and is induced during multicellular development with kinetics similar to those of SP60 gene expression. This activity is also induced in response to cAMP and specifically binds the wild-type CAE-1- and CAE-2-containing oligonucleotides. CAE-1 and CAE-2 oligonucleotides containing point mutations within the CAE core sequence neither bind to nor compete for the cAMP-induced, developmentally regulated factor(s) and result in substantial reductions in expression levels when substituted for the wild-type CAEs in vivo. The correlation between in vitro binding and in vivo function suggests that the CAE-1/CAE-2 binding activity may be involved in regulating cAMP and developmentally induced expression of SP60. A second, constitutive in vitro binding activity with high affinity to CAE-3 is also described. Models are proposed to relate the binding activities with the effects of the mutations on the spatial patterning of SP60-lacZ expression."xsd:string |
| http://purl.uniprot.org/citations/1668278 | http://purl.org/dc/terms/identifier | "doi:10.1242/dev.113.3.947"xsd:string |
| http://purl.uniprot.org/citations/1668278 | http://purl.uniprot.org/core/author | "Firtel R.A."xsd:string |
| http://purl.uniprot.org/citations/1668278 | http://purl.uniprot.org/core/author | "Haberstroh L."xsd:string |
| http://purl.uniprot.org/citations/1668278 | http://purl.uniprot.org/core/author | "Galindo J."xsd:string |
| http://purl.uniprot.org/citations/1668278 | http://purl.uniprot.org/core/date | "1991"xsd:gYear |
| http://purl.uniprot.org/citations/1668278 | http://purl.uniprot.org/core/name | "Development"xsd:string |
| http://purl.uniprot.org/citations/1668278 | http://purl.uniprot.org/core/pages | "947-958"xsd:string |
| http://purl.uniprot.org/citations/1668278 | http://purl.uniprot.org/core/title | "Developmental and spatial regulation of a Dictyostelium prespore gene: cis-acting elements and a cAMP-induced, developmentally regulated DNA binding activity."xsd:string |
| http://purl.uniprot.org/citations/1668278 | http://purl.uniprot.org/core/volume | "113"xsd:string |
| http://purl.uniprot.org/citations/1668278 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/1668278 |
| http://purl.uniprot.org/citations/1668278 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/1668278 |
| http://purl.uniprot.org/uniprot/#_P15270-mappedCitation-1668278 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/1668278 |
| http://purl.uniprot.org/uniprot/#_P36417-mappedCitation-1668278 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/1668278 |
| http://purl.uniprot.org/uniprot/P15270 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/1668278 |
| http://purl.uniprot.org/uniprot/P36417 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/1668278 |