Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/16701625http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/16701625http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/16701625http://www.w3.org/2000/01/rdf-schema#comment"The C. elegans pharynx is produced from the embryonic blastomeres ABa and MS. Pharyngeal fate in the ABa lineage is specified by the combined activities of GLP-1/Notch-mediated signals and the TBX-37 and TBX-38 T-box transcription factors. Here, we show another T-box factor TBX-2 also functions in ABa-derived pharyngeal development. tbx-2 mutants arrest as L1 larvae lacking most or all ABa-derived pharyngeal muscles. In comparison, tbx-2 mutants retain ABa-derived marginal cells and pharyngeal muscles derived from MS. A tbx-2Colon, two colonsgfp translational fusion is expressed in a dynamic pattern in C. elegans embryos beginning near the 100-cell stage. Early expression is limited to a small number of cells, which likely include the ABa-derived pharyngeal precursors, while later expression is observed in body wall muscles and a subset of pharyngeal neurons. TBX-2 contains 2 consensus sumoylation sites, and it interacts in a yeast two-hybrid assay with the UBC-9 and GEI-17 components of the C. elegans SUMO-conjugating pathway. ubc-9(RNAi) has been previously shown to cause variable embryonic and larval arrest, and we find that, like tbx-2 mutants, ubc-9(RNAi) animals lack ABa-derived pharyngeal muscles. ubc-9(RNAi) also alters the subnuclear distribution of TBX-2::GFP fusion protein, suggesting that UBC-9 and TBX-2 interact in C. elegans. Together, these results indicate that TBX-2 and SUMO-conjugating enzymes are necessary for ABa-derived pharyngeal muscle, and we hypothesize that TBX-2 function requires sumoylation. Sumoylation is increasingly recognized as an important mechanism controlling activity of many nuclear factors, and these results provide the first evidence that T-box factor activity may require sumoylation."xsd:string
http://purl.uniprot.org/citations/16701625http://purl.org/dc/terms/identifier"doi:10.1016/j.ydbio.2006.04.001"xsd:string
http://purl.uniprot.org/citations/16701625http://purl.org/dc/terms/identifier"doi:10.1016/j.ydbio.2006.04.001"xsd:string
http://purl.uniprot.org/citations/16701625http://purl.uniprot.org/core/author"Woollard A."xsd:string
http://purl.uniprot.org/citations/16701625http://purl.uniprot.org/core/author"Woollard A."xsd:string
http://purl.uniprot.org/citations/16701625http://purl.uniprot.org/core/author"Okkema P.G."xsd:string
http://purl.uniprot.org/citations/16701625http://purl.uniprot.org/core/author"Okkema P.G."xsd:string
http://purl.uniprot.org/citations/16701625http://purl.uniprot.org/core/author"Aslam S."xsd:string
http://purl.uniprot.org/citations/16701625http://purl.uniprot.org/core/author"Aslam S."xsd:string
http://purl.uniprot.org/citations/16701625http://purl.uniprot.org/core/author"Crum T."xsd:string
http://purl.uniprot.org/citations/16701625http://purl.uniprot.org/core/author"Crum T."xsd:string
http://purl.uniprot.org/citations/16701625http://purl.uniprot.org/core/author"Roy Chowdhuri S."xsd:string
http://purl.uniprot.org/citations/16701625http://purl.uniprot.org/core/author"Roy Chowdhuri S."xsd:string
http://purl.uniprot.org/citations/16701625http://purl.uniprot.org/core/date"2006"xsd:gYear
http://purl.uniprot.org/citations/16701625http://purl.uniprot.org/core/date"2006"xsd:gYear
http://purl.uniprot.org/citations/16701625http://purl.uniprot.org/core/name"Dev. Biol."xsd:string
http://purl.uniprot.org/citations/16701625http://purl.uniprot.org/core/name"Dev. Biol."xsd:string
http://purl.uniprot.org/citations/16701625http://purl.uniprot.org/core/pages"664-677"xsd:string
http://purl.uniprot.org/citations/16701625http://purl.uniprot.org/core/pages"664-677"xsd:string
http://purl.uniprot.org/citations/16701625http://purl.uniprot.org/core/title"The T-box factor TBX-2 and the SUMO conjugating enzyme UBC-9 are required for ABa-derived pharyngeal muscle in C. elegans."xsd:string
http://purl.uniprot.org/citations/16701625http://purl.uniprot.org/core/title"The T-box factor TBX-2 and the SUMO conjugating enzyme UBC-9 are required for ABa-derived pharyngeal muscle in C. elegans."xsd:string
http://purl.uniprot.org/citations/16701625http://purl.uniprot.org/core/volume"295"xsd:string
http://purl.uniprot.org/citations/16701625http://purl.uniprot.org/core/volume"295"xsd:string
▶