| http://purl.uniprot.org/citations/16701907 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16701907 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16701907 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16701907 | http://www.w3.org/2000/01/rdf-schema#comment | "Full-length HIV-1 genome sequencing provides important data needed to address several vaccine design, molecular epidemiologic and pathogenesis questions. A protocol is presented for obtaining near full-length genomes (NFLGs) from subjects infected with HIV-1 subtype C. This protocol was used to amplify NFLGs from 244 of 366 (67%) samples collected at two clinics in Durban, South Africa (SK and PS). Viral load was directly associated with frequency of successful NFLG amplification for both cohorts (PS; p = 0.005 and SK; p < 0.001). Seventeen of 38 initially NFLG-negative SK samples had variation within the PCR primer binding sites, however only 3 of these were successfully re-amplified using re-designed primers homologous to the target viruses. NFLGs were obtained from 7 of 24 PBMC samples processed from subjects whose plasma did not yield a NFLG. Stable plasmid clones were obtained from all 244 NFLG-positive PCR products, and both strands of each genome were sequenced, using a primary set of 46 primers. These methods thus allow the large-scale collection of HIV-1 NFLGs from populations infected primarily with subtype C. The methods are readily adaptable to other HIV-1 subtypes, and provide materials for viral functional analyses and population-based molecular epidemiology studies that include analysis of viral genome chimerization."xsd:string |
| http://purl.uniprot.org/citations/16701907 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.jviromet.2006.04.009"xsd:string |
| http://purl.uniprot.org/citations/16701907 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.jviromet.2006.04.009"xsd:string |
| http://purl.uniprot.org/citations/16701907 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.jviromet.2006.04.009"xsd:string |
| http://purl.uniprot.org/citations/16701907 | http://purl.uniprot.org/core/author | "McKay A.R."xsd:string |
| http://purl.uniprot.org/citations/16701907 | http://purl.uniprot.org/core/author | "McKay A.R."xsd:string |
| http://purl.uniprot.org/citations/16701907 | http://purl.uniprot.org/core/author | "McKay A.R."xsd:string |
| http://purl.uniprot.org/citations/16701907 | http://purl.uniprot.org/core/author | "McLaughlin S."xsd:string |
| http://purl.uniprot.org/citations/16701907 | http://purl.uniprot.org/core/author | "McLaughlin S."xsd:string |
| http://purl.uniprot.org/citations/16701907 | http://purl.uniprot.org/core/author | "McLaughlin S."xsd:string |
| http://purl.uniprot.org/citations/16701907 | http://purl.uniprot.org/core/author | "Mullins J.I."xsd:string |
| http://purl.uniprot.org/citations/16701907 | http://purl.uniprot.org/core/author | "Mullins J.I."xsd:string |
| http://purl.uniprot.org/citations/16701907 | http://purl.uniprot.org/core/author | "Mullins J.I."xsd:string |
| http://purl.uniprot.org/citations/16701907 | http://purl.uniprot.org/core/author | "Walker B.D."xsd:string |
| http://purl.uniprot.org/citations/16701907 | http://purl.uniprot.org/core/author | "Walker B.D."xsd:string |
| http://purl.uniprot.org/citations/16701907 | http://purl.uniprot.org/core/author | "Walker B.D."xsd:string |
| http://purl.uniprot.org/citations/16701907 | http://purl.uniprot.org/core/author | "Learn G.H."xsd:string |
| http://purl.uniprot.org/citations/16701907 | http://purl.uniprot.org/core/author | "Learn G.H."xsd:string |
| http://purl.uniprot.org/citations/16701907 | http://purl.uniprot.org/core/author | "Learn G.H."xsd:string |
| http://purl.uniprot.org/citations/16701907 | http://purl.uniprot.org/core/author | "Lee T.C."xsd:string |
| http://purl.uniprot.org/citations/16701907 | http://purl.uniprot.org/core/author | "Lee T.C."xsd:string |
| http://purl.uniprot.org/citations/16701907 | http://purl.uniprot.org/core/author | "Lee T.C."xsd:string |