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http://purl.uniprot.org/citations/16703469 | http://www.w3.org/2000/01/rdf-schema#comment | "The interaction of human visinin-like protein 1 (VILIP1) and visinin-like protein 3 (VILIP3) with divalent cations (Mg2+, Ca2+, Sr2+ and Ba2+) was explored using circular dichroism and fluorescence measurement. These results showed that the four cations each induced a different subtle change in the conformation of VILIPs. Moreover, VILIP1 and VILIP3 bound with Ca2+ or Mg2+ in a cooperative manner. Studies on the truncated mutants showed that the intact EF-3 and EF-4 were essential for the binding of VILIP1 with Ca2+ and Mg2+. Pull-down assay revealed that Ca2+ and Mg2+ enhanced the intermolecular interaction of VILIPs, and led to the formation of homo- and hetero-oligomer of VILIPs. Together with previous findings that Ca2+-dependent localization of VILIPs may be involved in the regulation of distinct cascades and deprivation of Ca2+-binding capacity of VILIPs did not completely eliminate their activity, it is likely to reflect that Mg2+-bound VILIPs may play a role in regulating the biological function of VILIPs in response to a concentration fluctuation of Ca2+ in cells."xsd:string |
http://purl.uniprot.org/citations/16703469 | http://purl.org/dc/terms/identifier | "doi:10.1007/s10930-006-9008-5"xsd:string |
http://purl.uniprot.org/citations/16703469 | http://purl.uniprot.org/core/author | "Chang L.S."xsd:string |
http://purl.uniprot.org/citations/16703469 | http://purl.uniprot.org/core/author | "Lee L."xsd:string |
http://purl.uniprot.org/citations/16703469 | http://purl.uniprot.org/core/author | "Wang L."xsd:string |
http://purl.uniprot.org/citations/16703469 | http://purl.uniprot.org/core/author | "Jheng F.F."xsd:string |
http://purl.uniprot.org/citations/16703469 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
http://purl.uniprot.org/citations/16703469 | http://purl.uniprot.org/core/name | "Protein J"xsd:string |
http://purl.uniprot.org/citations/16703469 | http://purl.uniprot.org/core/pages | "250-256"xsd:string |
http://purl.uniprot.org/citations/16703469 | http://purl.uniprot.org/core/title | "Functional contribution of Ca2+ and Mg2+ to the intermolecular interaction of visinin-like proteins."xsd:string |
http://purl.uniprot.org/citations/16703469 | http://purl.uniprot.org/core/volume | "25"xsd:string |
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