| http://purl.uniprot.org/citations/16730023 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16730023 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16730023 | http://www.w3.org/2000/01/rdf-schema#comment | "The novel flavin-dependent thymidylate synthase, ThyX, is absent in humans but several pathogenic bacteria depend exclusively on ThyX activity to synthesize thymidylate. Reduction of the enzyme-bound FAD by NADPH is suggested to be the critical first step in ThyX catalysis. We soaked Mycobacterium tuberculosis ThyX-FAD-BrdUMP ternary complex crystals in a solution containing NADP+ to gain structural insights into the reductive step of the catalytic cycle. Surprisingly, the NADP+ displaced both FAD and BrdUMP from the active site. In the resultant ThyX-NADP+ binary complex, the AMP moiety is bound in a deep pocket similar to that of the same moiety of FAD in the ternary complex, while the nicotinamide part of NADP+ is engaged in a limited number of contacts with ThyX. The additional 2'-phosphate group attached to the AMP ribose of NADP+ could be accommodated with minor rearrangement of water molecules. The newly introduced 2'-phosphate groups are engaged in water-mediated interactions across the non-crystallographic 2-fold axis of the ThyX tetramer, suggesting possibilities for design of high-affinity bivalent inhibitors of this intriguing enzyme."xsd:string |
| http://purl.uniprot.org/citations/16730023 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.jmb.2006.04.061"xsd:string |
| http://purl.uniprot.org/citations/16730023 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.jmb.2006.04.061"xsd:string |
| http://purl.uniprot.org/citations/16730023 | http://purl.uniprot.org/core/author | "Hol W.G."xsd:string |
| http://purl.uniprot.org/citations/16730023 | http://purl.uniprot.org/core/author | "Hol W.G."xsd:string |
| http://purl.uniprot.org/citations/16730023 | http://purl.uniprot.org/core/author | "Sampathkumar P."xsd:string |
| http://purl.uniprot.org/citations/16730023 | http://purl.uniprot.org/core/author | "Sampathkumar P."xsd:string |
| http://purl.uniprot.org/citations/16730023 | http://purl.uniprot.org/core/author | "Turley S."xsd:string |
| http://purl.uniprot.org/citations/16730023 | http://purl.uniprot.org/core/author | "Turley S."xsd:string |
| http://purl.uniprot.org/citations/16730023 | http://purl.uniprot.org/core/author | "Sibley C.H."xsd:string |
| http://purl.uniprot.org/citations/16730023 | http://purl.uniprot.org/core/author | "Sibley C.H."xsd:string |
| http://purl.uniprot.org/citations/16730023 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16730023 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16730023 | http://purl.uniprot.org/core/name | "J. Mol. Biol."xsd:string |
| http://purl.uniprot.org/citations/16730023 | http://purl.uniprot.org/core/name | "J. Mol. Biol."xsd:string |
| http://purl.uniprot.org/citations/16730023 | http://purl.uniprot.org/core/pages | "1-6"xsd:string |
| http://purl.uniprot.org/citations/16730023 | http://purl.uniprot.org/core/pages | "1-6"xsd:string |
| http://purl.uniprot.org/citations/16730023 | http://purl.uniprot.org/core/title | "NADP+ expels both the co-factor and a substrate analog from the Mycobacterium tuberculosis ThyX active site: opportunities for anti-bacterial drug design."xsd:string |
| http://purl.uniprot.org/citations/16730023 | http://purl.uniprot.org/core/title | "NADP+ expels both the co-factor and a substrate analog from the Mycobacterium tuberculosis ThyX active site: opportunities for anti-bacterial drug design."xsd:string |
| http://purl.uniprot.org/citations/16730023 | http://purl.uniprot.org/core/volume | "360"xsd:string |
| http://purl.uniprot.org/citations/16730023 | http://purl.uniprot.org/core/volume | "360"xsd:string |
| http://purl.uniprot.org/citations/16730023 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16730023 |
| http://purl.uniprot.org/citations/16730023 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16730023 |