| http://purl.uniprot.org/citations/16733250 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16733250 | http://www.w3.org/2000/01/rdf-schema#comment | "Minibrain kinase/dual-specificity tyrosine phosphorylation-regulated kinase (Mnb/Dyrk1A) is a proline-directed serine/threonine kinase encoded in the Down syndrome critical region of human chromosome 21. This kinase has been shown to phosphorylate dynamin 1 and synaptojanin 1. Here we report that amphiphysin I (Amph I) is also a Mnb/Dyrk1A substrate. This kinase phosphorylated native Amph I in rodent brains and recombinant human Amph I expressed in Escherichia coli. Serine 293 (Ser-293) was identified as the major site, whereas serine 295 and threonine 310 were found as minor kinase sites. In cultured cells, recombinant Amph I was phosphorylated at Ser-293 by endogenous kinase(s). Because mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) has been suggested to phosphorylate Amph I at Ser-293, our efforts addressed whether Ser-293 is phosphorylated in vivo by MAPK/ERK or by Mnb/Dyrk1A. Overnight serum-withdrawal inactivated MAPK/ERK; nonetheless, Ser-293 was phosphorylated in Chinese hamster ovary and SY5Y cells. Epigallocatechin-3-gallate, a potent Mnb/Dyrk1A inhibitor in vitro, apparently reduced the phosphorylation at Ser-293, whereas PD98059, a potent MAPK/ERK inhibitor, did not. High frequency stimulation of mouse hippocampal slices reduced the phosphorylation at Ser-293, albeit in the midst of MAPK/ERK activation. The endophilin binding in vitro was inhibited by phosphorylating Amph I with Mnb/Dyrk1A. However, phosphorylation at Ser-293 did not appear to alter cellular distribution patterns of the protein. Our results suggest that Mnb/Dyrk1A, not MAPK/ERK, is responsible for in vivo phosphorylation of Amph I at Ser-293 and that phosphorylation changes the recruitment of endophilin at the endocytic sites."xsd:string |
| http://purl.uniprot.org/citations/16733250 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m513497200"xsd:string |
| http://purl.uniprot.org/citations/16733250 | http://purl.uniprot.org/core/author | "Xie W."xsd:string |
| http://purl.uniprot.org/citations/16733250 | http://purl.uniprot.org/core/author | "Lu R.C."xsd:string |
| http://purl.uniprot.org/citations/16733250 | http://purl.uniprot.org/core/author | "Murakami N."xsd:string |
| http://purl.uniprot.org/citations/16733250 | http://purl.uniprot.org/core/author | "Wieraszko A."xsd:string |
| http://purl.uniprot.org/citations/16733250 | http://purl.uniprot.org/core/author | "Hwang Y.W."xsd:string |
| http://purl.uniprot.org/citations/16733250 | http://purl.uniprot.org/core/author | "Chen-Hwang M.C."xsd:string |
| http://purl.uniprot.org/citations/16733250 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16733250 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/16733250 | http://purl.uniprot.org/core/pages | "23712-23724"xsd:string |
| http://purl.uniprot.org/citations/16733250 | http://purl.uniprot.org/core/title | "Phosphorylation of amphiphysin I by minibrain kinase/dual-specificity tyrosine phosphorylation-regulated kinase, a kinase implicated in Down syndrome."xsd:string |
| http://purl.uniprot.org/citations/16733250 | http://purl.uniprot.org/core/volume | "281"xsd:string |
| http://purl.uniprot.org/citations/16733250 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16733250 |
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| http://purl.uniprot.org/uniprot/#_A8K5Y2-mappedCitation-16733250 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16733250 |
| http://purl.uniprot.org/uniprot/#_P49418-mappedCitation-16733250 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16733250 |
| http://purl.uniprot.org/uniprot/#_Q8NFL3-mappedCitation-16733250 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16733250 |
| http://purl.uniprot.org/uniprot/#_Q8NFL4-mappedCitation-16733250 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16733250 |
| http://purl.uniprot.org/uniprot/#_Q8NFL5-mappedCitation-16733250 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16733250 |
| http://purl.uniprot.org/uniprot/#_Q8NFL6-mappedCitation-16733250 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16733250 |
| http://purl.uniprot.org/uniprot/#_Q8NFL7-mappedCitation-16733250 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16733250 |
| http://purl.uniprot.org/uniprot/#_Q8NFL8-mappedCitation-16733250 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16733250 |
| http://purl.uniprot.org/uniprot/#_Q9UQI2-mappedCitation-16733250 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16733250 |