| http://purl.uniprot.org/citations/16737959 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16737959 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16737959 | http://www.w3.org/2000/01/rdf-schema#comment | "Hic-5 is a focal adhesion LIM protein serving as a scaffold in integrin signaling. The protein comprises four LD domains in its N-terminal half and four LIM domains in its C-terminal half with a nuclear export signal in LD3 and is shuttled between the cytoplasmic and nuclear compartments. In this study, immunoprecipitation and in vitro cross-linking experiments showed that Hic-5 homo-oligomerized through its most C-terminal LIM domain, LIM4. Strikingly, paxillin, the protein most homologous to Hic-5, did not show this capability. Gel filtration analysis also revealed that Hic-5 differs from paxillin in that it has multiple forms in the cellular environment, and Hic-5 but not paxillin was capable of hetero-oligomerization with a LIM-only protein, PINCH, another molecular scaffold at focal adhesions. The fourth LIM domain of Hic-5 and the fifth LIM domain region of PINCH constituted the interface for the interaction. The complex included integrin-linked kinase, a binding partner of PINCH, which also interacted with Hic-5 through the region encompassing the pleckstrin homology-like domain and LIM domains of Hic-5. Of note, Hic-5 marginally affected the subcellular distribution of PINCH but directed its shuttling between the cytoplasmic and nuclear compartments in the presence of integrin-linked kinase. Uncoupling of the two signaling platforms of Hic-5 and PINCH through interference with the hetero-oligomerization resulted in impairment of cellular growth. Hic-5 is, thus, a molecular scaffold with the potential to dock with another scaffold through the LIM domain, organizing a mobile supramolecular unit and coordinating the adhesion signal with cellular activities in the two compartments."xsd:string |
| http://purl.uniprot.org/citations/16737959 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m513111200"xsd:string |
| http://purl.uniprot.org/citations/16737959 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m513111200"xsd:string |
| http://purl.uniprot.org/citations/16737959 | http://purl.uniprot.org/core/author | "Hayashi M."xsd:string |
| http://purl.uniprot.org/citations/16737959 | http://purl.uniprot.org/core/author | "Hayashi M."xsd:string |
| http://purl.uniprot.org/citations/16737959 | http://purl.uniprot.org/core/author | "Mori K."xsd:string |
| http://purl.uniprot.org/citations/16737959 | http://purl.uniprot.org/core/author | "Mori K."xsd:string |
| http://purl.uniprot.org/citations/16737959 | http://purl.uniprot.org/core/author | "Ohki T."xsd:string |
| http://purl.uniprot.org/citations/16737959 | http://purl.uniprot.org/core/author | "Ohki T."xsd:string |
| http://purl.uniprot.org/citations/16737959 | http://purl.uniprot.org/core/author | "Asakawa M."xsd:string |
| http://purl.uniprot.org/citations/16737959 | http://purl.uniprot.org/core/author | "Asakawa M."xsd:string |
| http://purl.uniprot.org/citations/16737959 | http://purl.uniprot.org/core/author | "Nose K."xsd:string |
| http://purl.uniprot.org/citations/16737959 | http://purl.uniprot.org/core/author | "Nose K."xsd:string |
| http://purl.uniprot.org/citations/16737959 | http://purl.uniprot.org/core/author | "Kim-Kaneyama J.-R."xsd:string |
| http://purl.uniprot.org/citations/16737959 | http://purl.uniprot.org/core/author | "Kim-Kaneyama J.-R."xsd:string |
| http://purl.uniprot.org/citations/16737959 | http://purl.uniprot.org/core/author | "Shibanuma M."xsd:string |
| http://purl.uniprot.org/citations/16737959 | http://purl.uniprot.org/core/author | "Shibanuma M."xsd:string |
| http://purl.uniprot.org/citations/16737959 | http://purl.uniprot.org/core/author | "Hirao E."xsd:string |
| http://purl.uniprot.org/citations/16737959 | http://purl.uniprot.org/core/author | "Hirao E."xsd:string |
| http://purl.uniprot.org/citations/16737959 | http://purl.uniprot.org/core/author | "Imura M."xsd:string |
| http://purl.uniprot.org/citations/16737959 | http://purl.uniprot.org/core/author | "Imura M."xsd:string |
| http://purl.uniprot.org/citations/16737959 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16737959 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |