| http://purl.uniprot.org/citations/16750518 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16750518 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16750518 | http://www.w3.org/2000/01/rdf-schema#comment | "A novel acidic Asp49 phospholipase A(2) was isolated from Bothrops erythromelas (jararaca malha-de-cascavel) snake venom by four chromatographic steps. BE-I-PLA2 present a molecular weight of 13,649.57 Da as estimated by mass spectrometry. N-terminal and four internal peptides were sequenced, covering around one-third of the complete toxin sequence. The complete BE-I-PLA2 cDNA was cloned from a B. erythromelas venom-gland cDNA library. The cDNA sequence possesses 457 bp and encodes a protein with significant sequence similarity to many other phospholipase A(2) from snake venoms. When tested in platelet rich plasma, the enzyme showed a potent inhibitory effect on aggregation induced by arachidonic acid and collagen, but not ADP. On the other hand, BE-I-PLA2 did not modify aggregation in washed platelet. Furthermore, no action of BE-I-PLA2 on the principal platelets receptors was observed. Chemical modification with p-bromophenacyl bromide abolished the enzymatic activity of BE-I-PLA2, but its anti-platelet activity was only partially inhibited. In human umbilical-cord veins endothelial cells, BE-I-PLA2 was neither apoptotic nor proliferative but stimulated endothelial cells to release prostaglandin I(2), suggesting an increase of its potential anti-platelet activity in vivo. Further studies are required in order to determine the exact mechanism of action of BE-I-PLA2 in the inhibition of platelet aggregation."xsd:string |
| http://purl.uniprot.org/citations/16750518 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bcp.2006.04.032"xsd:string |
| http://purl.uniprot.org/citations/16750518 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bcp.2006.04.032"xsd:string |
| http://purl.uniprot.org/citations/16750518 | http://purl.uniprot.org/core/author | "Oliva M.L."xsd:string |
| http://purl.uniprot.org/citations/16750518 | http://purl.uniprot.org/core/author | "Oliva M.L."xsd:string |
| http://purl.uniprot.org/citations/16750518 | http://purl.uniprot.org/core/author | "Spencer P.J."xsd:string |
| http://purl.uniprot.org/citations/16750518 | http://purl.uniprot.org/core/author | "Spencer P.J."xsd:string |
| http://purl.uniprot.org/citations/16750518 | http://purl.uniprot.org/core/author | "Chudzinski-Tavassi A.M."xsd:string |
| http://purl.uniprot.org/citations/16750518 | http://purl.uniprot.org/core/author | "Chudzinski-Tavassi A.M."xsd:string |
| http://purl.uniprot.org/citations/16750518 | http://purl.uniprot.org/core/author | "Fritzen M."xsd:string |
| http://purl.uniprot.org/citations/16750518 | http://purl.uniprot.org/core/author | "Fritzen M."xsd:string |
| http://purl.uniprot.org/citations/16750518 | http://purl.uniprot.org/core/author | "Guarnieri M.C."xsd:string |
| http://purl.uniprot.org/citations/16750518 | http://purl.uniprot.org/core/author | "Guarnieri M.C."xsd:string |
| http://purl.uniprot.org/citations/16750518 | http://purl.uniprot.org/core/author | "Valenca R.C."xsd:string |
| http://purl.uniprot.org/citations/16750518 | http://purl.uniprot.org/core/author | "Valenca R.C."xsd:string |
| http://purl.uniprot.org/citations/16750518 | http://purl.uniprot.org/core/author | "da Silva M.B."xsd:string |
| http://purl.uniprot.org/citations/16750518 | http://purl.uniprot.org/core/author | "da Silva M.B."xsd:string |
| http://purl.uniprot.org/citations/16750518 | http://purl.uniprot.org/core/author | "de Albuquerque Modesto J.C."xsd:string |
| http://purl.uniprot.org/citations/16750518 | http://purl.uniprot.org/core/author | "de Albuquerque Modesto J.C."xsd:string |
| http://purl.uniprot.org/citations/16750518 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16750518 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16750518 | http://purl.uniprot.org/core/name | "Biochem. Pharmacol."xsd:string |
| http://purl.uniprot.org/citations/16750518 | http://purl.uniprot.org/core/name | "Biochem. Pharmacol."xsd:string |