| http://purl.uniprot.org/citations/16760379 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16760379 | http://www.w3.org/2000/01/rdf-schema#comment | "We recently identified a differentially expressed gene in implantation stage rabbit endometrium encoding a new member of the ubiquitin-conjugating enzyme family designated UBE2Q2 (also known as UBCi). Its unusually high molecular mass, novel N-terminus extension, and highly selective pattern of mRNA expression suggest a specific function in implantation. This study analyzes its relationship to the E2 ubiquitin-conjugating enzyme superfamily, investigates its enzymatic activity, and examines its localization in implantation site endometrium. Construction of a dendrogram indicated that UBE2Q2 is homologous to the UBC2 family of enzymes, and isoforms are present in a broad range of species. In vitro enzymatic assays of ubiquitin thiolester formation demonstrated that UBE2Q2 is a functional ubiquitin-conjugating enzyme. The Km for transfer of ubiquitin thiolester from E1 to UBE2Q2 is 817 nM compared to 100 nM for other E2 paralogs; this suggests that the unique amino terminal domain of UBE2Q2 confers specific functional differences. Affinity-purified antibodies prepared with purified recombinant UBE2Q2 showed that the protein was undetectable by immunoblot analysis in endometrial lysates from estrous and Day 6(3/4) pregnant (blastocyst attachment stage) rabbits but was expressed in both mesometrial and antimesometrial implantation site endometrium of Day 8 pregnant animals. No expression was detected in adjacent interimplantion sites. Immunohistochemistry demonstrated UBE2Q2 expression exclusively in mesometrial and antimesometrial endometrial luminal epithelial cells of the Day 8 implantation chamber. Immunohistochemical localization of ubiquitin mirrored UBE2Q2 expression, with low-to-undetectable levels in implantation sites of Day 6(3/4) pregnant endometrium but high levels in luminal epithelial cells of Day 8 pregnant endometrium. This implantation site-specific expression of UBE2Q2 in luminal epithelial cells could play major roles in orchestrating differentiation events through the modification of specific protein substrates."xsd:string |
| http://purl.uniprot.org/citations/16760379 | http://purl.org/dc/terms/identifier | "doi:10.1095/biolreprod.106.051458"xsd:string |
| http://purl.uniprot.org/citations/16760379 | http://purl.uniprot.org/core/author | "Melner M.H."xsd:string |
| http://purl.uniprot.org/citations/16760379 | http://purl.uniprot.org/core/author | "Olson G.E."xsd:string |
| http://purl.uniprot.org/citations/16760379 | http://purl.uniprot.org/core/author | "Winfrey V.P."xsd:string |
| http://purl.uniprot.org/citations/16760379 | http://purl.uniprot.org/core/author | "Boeglin W.E."xsd:string |
| http://purl.uniprot.org/citations/16760379 | http://purl.uniprot.org/core/author | "Brash A.R."xsd:string |
| http://purl.uniprot.org/citations/16760379 | http://purl.uniprot.org/core/author | "Haas A.L."xsd:string |
| http://purl.uniprot.org/citations/16760379 | http://purl.uniprot.org/core/author | "Klein J.M."xsd:string |
| http://purl.uniprot.org/citations/16760379 | http://purl.uniprot.org/core/author | "Nagdas S.K."xsd:string |
| http://purl.uniprot.org/citations/16760379 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16760379 | http://purl.uniprot.org/core/name | "Biol Reprod"xsd:string |
| http://purl.uniprot.org/citations/16760379 | http://purl.uniprot.org/core/pages | "395-406"xsd:string |
| http://purl.uniprot.org/citations/16760379 | http://purl.uniprot.org/core/title | "Demonstration of ubiquitin thiolester formation of UBE2Q2 (UBCi), a novel ubiquitin-conjugating enzyme with implantation site-specific expression."xsd:string |
| http://purl.uniprot.org/citations/16760379 | http://purl.uniprot.org/core/volume | "75"xsd:string |
| http://purl.uniprot.org/citations/16760379 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16760379 |
| http://purl.uniprot.org/citations/16760379 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/16760379 |
| http://purl.uniprot.org/uniprot/Q8K2Z8#attribution-8E2299A8627C94687E9A15DD80D9232D | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/16760379 |
| http://purl.uniprot.org/uniprot/#_D3Z647-mappedCitation-16760379 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16760379 |
| http://purl.uniprot.org/uniprot/#_Q8K2Z8-mappedCitation-16760379 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16760379 |
| http://purl.uniprot.org/uniprot/Q8K2Z8 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/16760379 |
| http://purl.uniprot.org/uniprot/D3Z647 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/16760379 |